Analgesic Effect of Sulforaphane: A New Application for Poloxamer-Hyaluronic Acid Hydrogels

Autor(es)

Papini, Juliana Zampoli Boava ; Esteves, Bruno de Assis ; Oliveira, Vagner Gomes de Souza ; Abdalla, Henrique Ballassani ; Cereda, Cintia Maria Saia ; de Araujo, Daniele Ribeiro ; Tofoli, Giovana Radomille

• Tipo de documento: Artigo Científico
• Fonte: GELS; v. 10, n. 7, p. 14-pg., 2024-07-01.

Resumo

Sulforaphane (SFN) has shown potential as an antioxidant and anti-inflammatory agent. To improve its druggability, we developed new analgesic formulations with sulforaphane-loaded hyaluronic acid (HA)-poloxamer (PL) hydrogel. This study evaluated the pre-clinical safety and effectiveness of these formulations. Effectiveness was tested on Wistar rats divided into groups (n = 15) receiving (IM, 10 mg/kg) SFN formulations or control groups (without SFN). This study used a hind paw incision postoperative pain model to evaluate mechanical hypersensitivity with von Frey filaments. TNF-alpha, IL-1 beta, substance P, and CGRP levels verified anti-inflammatory activity in the hind paw tissue. Histopathology of tissues surrounding the injection site was assessed after 2 and 7 days post-treatment. To corroborate drug safety, cell viability of 3T3 and RAW 264.7 cultures was assessed. Additionally, RAW 264.7 cultures primed with carrageenan evaluated nitric oxide (NO) levels. All animals exhibited post-incisional hypersensitivity, and F2 (PL 407/338 (18/2%) + HA 1% + SFN 0.1%) showed a longer analgesic effect (p < 0.05). F2 reduced TNF-alpha, IL-1 beta, and CGRP levels (p < 0.05). Histopathological evaluation showed mild to moderate inflammatory reactions after the formulations' injections. F2 produced no significant difference in cell viability (p > 0.05) but reduced NO production (p < 0.05). Thus, our results highlight the biocompatibility and effectiveness of F2. (AU)

• Processo FAPESP: 22/13470-4 - Efeito de fotobiomodulação na cicatrização: estudo in vitro e in vivo
• Beneficiário: Giovana Tofoli Moniz
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/06969-0 - Avaliação pré-clínica de hidrogéis de poloxamer-ácido hialurônico para liberação de sulforafano no controle de dor pós-operatória
• Beneficiário: Giovana Tofoli Moniz
• Modalidade de apoio: Auxílio à Pesquisa - Regular