| Texto completo | |
| Autor(es): |
Papini, Juliana Zampoli Boava
;
Esteves, Bruno de Assis
;
Oliveira, Vagner Gomes de Souza
;
Abdalla, Henrique Ballassani
;
Cereda, Cintia Maria Saia
;
de Araujo, Daniele Ribeiro
;
Tofoli, Giovana Radomille
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | GELS; v. 10, n. 7, p. 14-pg., 2024-07-01. |
| Resumo | |
Sulforaphane (SFN) has shown potential as an antioxidant and anti-inflammatory agent. To improve its druggability, we developed new analgesic formulations with sulforaphane-loaded hyaluronic acid (HA)-poloxamer (PL) hydrogel. This study evaluated the pre-clinical safety and effectiveness of these formulations. Effectiveness was tested on Wistar rats divided into groups (n = 15) receiving (IM, 10 mg/kg) SFN formulations or control groups (without SFN). This study used a hind paw incision postoperative pain model to evaluate mechanical hypersensitivity with von Frey filaments. TNF-alpha, IL-1 beta, substance P, and CGRP levels verified anti-inflammatory activity in the hind paw tissue. Histopathology of tissues surrounding the injection site was assessed after 2 and 7 days post-treatment. To corroborate drug safety, cell viability of 3T3 and RAW 264.7 cultures was assessed. Additionally, RAW 264.7 cultures primed with carrageenan evaluated nitric oxide (NO) levels. All animals exhibited post-incisional hypersensitivity, and F2 (PL 407/338 (18/2%) + HA 1% + SFN 0.1%) showed a longer analgesic effect (p < 0.05). F2 reduced TNF-alpha, IL-1 beta, and CGRP levels (p < 0.05). Histopathological evaluation showed mild to moderate inflammatory reactions after the formulations' injections. F2 produced no significant difference in cell viability (p > 0.05) but reduced NO production (p < 0.05). Thus, our results highlight the biocompatibility and effectiveness of F2. (AU) | |
| Processo FAPESP: | 22/13470-4 - Efeito de fotobiomodulação na cicatrização: estudo in vitro e in vivo |
| Beneficiário: | Giovana Tofoli Moniz |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 19/06969-0 - Avaliação pré-clínica de hidrogéis de poloxamer-ácido hialurônico para liberação de sulforafano no controle de dor pós-operatória |
| Beneficiário: | Giovana Tofoli Moniz |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |