Exploring Bacterial Diversity of Endodontic Microbiota by Cloning and Sequencing 16S rRNA

Autor(es)

Ribeiro, Adriana C. ^[1] ; Matarazzo, Flavia ^[1] ; Faveri, Marcelo ^[2] ; Zezell, Denise M. ^[3] ; Mayer, Marcia P. A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Microbiol, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil

^[2] Univ Guarulhos, Dent Res Div, Dept Periodontol, Guarulhos - Brazil

^[3] IPEN CNEN SP, Dept Biophoton, Nucl & Energy Res Inst, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 37, n. 7, p. 922-926, JUL 2011.
• Citações Web of Science: 26

Resumo

Introduction: The characterization of microbial communities infecting the endodontic system in each clinical condition may help on the establishment of a correct prognosis and distinct strategies of treatment. The purpose of this study was to determine the bacterial diversity in primary endodontic infections by 16S ribosomal-RNA (rRNA) sequence analysis. Methods: Samples from root canals of untreated asymptomatic teeth (n = 12) exhibiting periapical lesions were obtained, 165 rRNA bacterial genomic libraries were constructed and sequenced, and bacterial diversity was estimated. Results: A total of 489 clones were analyzed (mean, 40.7 +/- 8.0 clones per sample). Seventy phylotypes were identified of which six were novel phylotypes belonging to the family Ruminococcaceae. The mean number of taxa per canal was 10.0, ranging from 3 to 21 per sample; 65.7% of the cloned sequences represented phylotypes for which no cultivated isolates have been reported. The most prevalent taxa were Atopobium rimae (50.0%), Dialister invisus, Pre-votella oris, Pseudoramibacter alactolyticus, and Tannerella forsythia (33.3%). Conclusions: Although several key species predominate in endodontic samples of asymptomatic cases with periapical lesions, the primary endodontic infection is characterized by a wide bacterial diversity, which is mostly represented by members of the phylum Firmicutes belonging to the class Clostridia followed by the phylum Bacteroidetes. (J Ended 2011;37:922-926) (AU)

• Processo FAPESP: 07/52492-3 - Avaliação microbiológica por análise molecular da terapia endodôntica associada à irradiação do canal radicular com laser diodo
• Beneficiário: Marcia Pinto Alves Mayer
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/59856-8 - Avaliação microbiológica por análise clonal de 16SrRNA da terapia endodôntica convencional e associada à irradiação do canal radicular com laser diodo
• Beneficiário: Adriana da Costa Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado