Tooth Tissue Engineering: Optimal Dental Stem Cell Harvest Based on Tooth Development

Autor(es)

Duailibi, Monica Talarico ^{[1, 2]} ; Duailibi, Silvio Eduardo ^{[1, 2]} ; Duailibi Neto, Eduardo Felippe ^[3] ; Negreiros, Renata Matalon ^[4] ; Jorge, Waldyr Antonio ^{[4, 3]} ; Ferreira, Lydia Masako ^[1] ; Vacanti, Joseph Phillip ^{[5, 6, 7]} ; Yelick, Pamela Crotty ^[8]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Plast Surg, UNIFESP CINTERGEN, Interdisciplinary Ctr Gene Therapy, Sao Paulo - Brazil

^[2] Natl Inst Sci & Technol, INCT Biofabris, Biofabricat Inst, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Dent, FFO FOUSP, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Oral Maxillofacial Surg Course Dent Sch, FFO FOUSP, Sao Paulo - Brazil

^[5] Harvard Univ, Sch Med, Boston, MA - USA

^[6] Massachusetts Gen Hosp, Lab Tissue Engn & Organ Fabricat, Boston, MA 02114 - USA

^[7] Dept Surg, Boston, MA - USA

^[8] Tufts Univ, Div Craniofacial & Mol Genet, Dept Oral & Maxillofacial Pathol, Boston, MA 02111 - USA

• Tipo de documento: Artigo Científico
• Fonte: ARTIFICIAL ORGANS; v. 35, n. 7, p. E129-E135, JUL 2011.
• Citações Web of Science: 9

Resumo

Our long-term objective is to devise reliable methods to generate biological replacement teeth exhibiting the physical properties and functions of naturally formed human teeth. Previously, we demonstrated the successful use of tissue engineering approaches to generate small, bioengineered tooth crowns from harvested pig and rat postnatal dental stem cells (DSCs). To facilitate characterizations of human DSCs, we have developed a novel radiographic staging system to accurately correlate human third molar tooth developmental stage with anticipated harvested DSC yield. Our results demonstrated that DSC yields were higher in less developed teeth (Stages 1 and 2), and lower in more developed teeth (Stages 3, 4, and 5). The greatest cell yields and colony-forming units (CFUs) capability was obtained from Stages 1 and 2 tooth dental pulp. We conclude that radiographic developmental staging can be used to accurately assess the utility of harvested human teeth for future dental tissue engineering applications. (AU)

• Processo FAPESP: 08/57860-3 - BIOFABRIS - Instituto de Biofabricação
• Beneficiário: Rubens Maciel Filho
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 07/51227-4 - Caracterização das células-tronco progenitoras de germes dentais humanos para a utilização em engenharia tecidual dos tecidos mineralizados, osso, cartilagem e dentes
• Beneficiário: Silvio Eduardo Duailibi
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 07/58856-7 - Criopreservacao de dentes humanos para a utilizacao de celulas tronco progenitoras dentais na construcao de substitutos biologicos.
• Beneficiário: Monica Talarico Duailibi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 07/59488-1 - Caracterizacao das celulas-tronco progenitoras de germes dentais humanos para a utilizacao em engenharia tecidual dos tecidos mineralizados, osso, cartilagem e dentes.
• Beneficiário: Silvio Eduardo Duailibi
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores