| Texto completo | |
| Autor(es): Mostrar menos - |
Floriano, Juliana Ferreira
;
Barbosa, Angelica Mercia Pascon
;
Emanueli, Costanza
;
de Lima, Patricia Rodrigues
;
de Oliveira, Rafael Guilen
;
De Carvalho, Carolina Neiva Frota
;
Floriano, Emerson Aparecido Ferreira
;
Zambuzzi, Willian Fernando
;
Pinto, Thais Silva
;
Fernandes, Fabio Henrique
;
Salvadori, Daisy Maria Favero
;
Magalhaes, Phillipe Franklin Coelho
;
Albano, Luiz Gustavo Simao
;
Graeff, Carlos Frederico de Oliveira
;
Brasil, Giovana Sant'Ana Pegorin
;
dos Santos, Lindomar Soares
;
Burd, Betina Sayeg
;
Cao, Wei
;
Herculano, Rondinelli Donizetti
;
Golim, Marjorie de Assis
;
Ferreira Junior, Rui Seabra
;
Sobrevia, Luis
;
Rudge, Marilza Vieira Cunha
Número total de Autores: 23
|
| Tipo de documento: | Artigo Científico |
| Fonte: | International Journal of Biological Macromolecules; v. 289, p. 14-pg., 2024-12-19. |
| Resumo | |
Women with gestational diabetes mellitus show a high risk of developing Gestational Diabetes Induced Myopathy (GDiM). GDiM is characterized by significant pelvic floor skeletal muscle atrophy and urinary incontinence. This study aimed to develop a natural rubber latex (NRL) based biodevice with mesenchymal/stromal stem cells (MSCs) for skeletal muscle regeneration for women with GDiM. NRL showed porosity, roughness, biocompatibility, and bioactivity. MSCs adhesion on the NRL scaffold surface was assessed by scanning electron microscopy (SEM), confocal microscopy, and zymography. The scaffold's physicochemical and biological properties were carried out by Fourier transform infrared spectroscopy (FTIR), swelling and degradation studies, hemolytic activity, and antioxidant activity (AA), using Electronic Paramagnetic Resonance (EPR). MSCs in culture expressed CD90, adhered to plastic, differentiated, and produced fibroblast colonies. A high rate of cell proliferation was seen in MSCs on the NRL scaffold. FTIR analysis confirmed protein structures and polyisoprene in the scaffold. Swelling and degradation showed low water uptake and weight loss. Furthermore, NRL presented a hemolytic rate of 2.90 +/- 0.26 % for 24 h, and EPR revealed the scaffold's strong AA. The generated biodevice has potential for muscle regeneration and may be useful as a therapeutic option for skeletal muscle disorders in GDiM or urinary incontinence. (AU) | |
| Processo FAPESP: | 16/01743-5 - Coorte da tríade gestacional: hiperglicemia, incontinência urinária e perfil clínico, molecular e ômico da miopatia hiperglicêmica na predição de incontinência e disfunção muscular e pesquisa translacional com biodevice para regeneração muscular em ratas |
| Beneficiário: | Marilza Vieira Cunha Rudge |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 17/21783-4 - Coorte da tríade gestacional: hiperglicemia, incontinência urinária e perfil clínico, molecular e ômico da miopatia hiperglicêmica na predição de incontinência e disfunção muscular e pesquisa translacional com biodevice para regeneração muscular em ratas |
| Beneficiário: | Juliana Ferreira Floriano |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 18/22406-2 - Exossomos em Diabetes Mellitus gestacional |
| Beneficiário: | Marilza Vieira Cunha Rudge |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |