Autor(es)

Ferreira, Daisy Camargo ; Sawazaki, Marina Ribeiro Batistuti ; Bachour Jr, Bassam ; Mulato, Marcelo

• Tipo de documento: Artigo Científico
• Fonte: BIOMEDICAL MICRODEVICES; v. 27, n. 1, p. 9-pg., 2025-03-01.

Resumo

The overexpression of Human Epidermal Growth Factor Receptor 2 (HER2) protein is specifically related to tumor cell proliferation in breast cancers. Its presence in biological serum samples indicates presence or progression of cancer, becoming a promise biomarker. However, their detection needs a simple and high accuracy platform. In this study, we report the develop and optimization of a simple highly sensitive electrochemical platform for HER2. Gold electrode surface was modified with a self-assembled monolayer composed by DNA aptamer, 6-(ferrocenyl) hexanethiol and 6-mercapto-1-hexanethiol. Electrochemical impedance spectroscopy was used to quantify the changes in capacitance on the interface due to the presence ferrocene, whether acting as a redox charge or its behavior under different HER2 concentration in PBS and undiluted human serum. As a result, the approach allows detection of HER2 with a limit of detection of 3.61 pg/mL, 12.28 nF sensitivity per decade and a linear range from 1 pM to 1 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\:\mu\:$$\end{document}M in serum. This electrochemical aptasensor can be applied to different arrays for aptamer screening and has a significant importance to interaction study of biological systems. (AU)

• Processo FAPESP: 13/26133-7 - Detecção de alterações relacionadas ao câncer de mama através de sensores de carga ou de massa acoplados a monocamadas de DNA
• Beneficiário: Marina Ribeiro Batistuti Sawazaki
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/13161-3 - Biossensores eletroquímicos para detecção da proteína HER2 relacionada ao câncer de mama
• Beneficiário: Daisy Camargo Ferreira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/24201-6 - Plataformas Microeletrônicas para Biossensores Eletroquímicos, Piezoelétricos e FETs
• Beneficiário: Marcelo Mulato
• Modalidade de apoio: Auxílio à Pesquisa - Regular