Immunohistochemical study of the expression of fatty acid synthase and Ki-67 in salivary gland tumors

Autor(es)

do Prado, Renata Falchete ^[1] ; da Silva Machado, Ana Lourdes ^[2] ; Dias Colombo, Carlos Eduardo ^[3] ; Carvalho, Yasmin Rodarte ^[2]

Afiliação do(s) autor(es)

^[1] Super Sch Cruzeiro, Dept Physiotherapy, BR-12245000 Sao Paulo - Brazil

^[2] Sao Paulo State Univ, Sao Jose dos Campos Dent Sch, Dept Biosci & Oral Diag, Sao Paulo - Brazil

^[3] Univ Taubate, Dept Dent, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ORAL PATHOLOGY & MEDICINE; v. 40, n. 6, p. 467-475, JUL 2011.
• Citações Web of Science: 7

Resumo

BACKGROUND: The enzyme fatty acid synthase plays a fundamental role in the biosynthesis of fatty acids. Several recent studies have demonstrated a high fatty acid synthase expression in malignant tumors. Few studies have been conducted in oral and salivary gland tumors describing the fatty acid synthase expression. METHODS: This study evaluated and compared, by immunohistochemical reaction, the expression of fatty acid synthase and Ki-67 in salivary gland tumors. The immunohistochemical study used the streptavidin-biotin-peroxidase technique, with antibodies anti-fatty acid synthase and anti-Ki-67. The fatty acid synthase was analyzed by scores, considering the intensity of labeling, quantity of labeled cells and histological component. The Ki-67 was analyzed by counting of one thousand cells, calculating the quantity of positive cells in regions with higher density of labeling. Statistical analysis was performed using the Pearson and Mann-Whitney correlation tests. RESULTS: There was greater fatty acid synthase expression in pleomorphic adenoma compared to other tumors, and predominance of Ki-67 in malignant tumors. Among these, the mucoepidermoid carcinoma presented the highest proliferation rate. The expression of fatty acid synthase and Ki-67 did not present correlation between the tumors analyzed, except in pleomorphic adenomas, with statistically significant relationship between them. CONCLUSION: It is suggested that the fat metabolism in salivary gland tumors is related to maintenance of cell differentiation. Its expression prevailed in benign tumors, while Ki-67 prevailed in the mucoepidermoid carcinoma, demonstrating its high proliferation rate, followed by the cystic adenoid carcinoma and polymorphous low grade adenocarcinoma. J Oral Pathol Med (2011) 40: 467-475 (AU)

• Processo FAPESP: 07/55604-7 - Estudo imunoistoquimico da expressao de acido graxo sintetase (fas) e ki-67 em neoplasias de glandulas salivares menores.
• Beneficiário: Yasmin Rodarte Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Regular