Systemic alpha-1 adrenergic receptor inhibition reduces sperm damage in adult and aging spontaneously hypertensive rats

Autor(es)

Machado, Nicolle R. ; Miyazaki, Mika A. ; Oliveira, Thayna D. ; Dias, Karina T. ; Colli, Lucas G. ; Belardin, Larissa B. ; Bertolla, Ricardo P. ; Rodrigues, Stephen F.

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 14, n. 1, p. 13-pg., 2024-11-20.

Resumo

Decreased sperm quality has been reported in men with different clinical conditions, including aging and hypertension. In the male reproductive tract, it has been suggested that the alpha 1-adrenergic receptor influences fertility and spermatogenesis, and important functions are also attributed to the renin-angiotensin axis, such as regulation of steroidogenesis, spermatogenesis, and sperm function. Previously, our group demonstrated impaired testicular vasomotion via alpha 1-adrenergic receptor activation and increased hypoxia-related proteins in the testes of spontaneously hypertensive rats (SHRs) compared to Wistar normotensive rats. In this study, we aimed to investigate the effect of hypertension and inhibition of systemic alpha 1-adrenergic receptor or angiotensin II AT1 receptor on sperm quality, sperm functional characteristics, and testicular microcirculation in rats from three different ages: young (8-10-week-old), adult (20-24-week-old) and older adult (60-66-week-old). We observed higher blood pressure in SHRs of all ages compared to age-matched Wistar rats. Lower blood pressure was observed either in prazosin or losartan-treated adult or aged SHRs. Additionally, lower sperm concentration, impaired motility and higher acrosome damage were demonstrated in SHRs. Prazosin treatment alleviated the effects of hypertension on sperm concentration and motility but not acrosome damage. Higher vasomotion was noticed in testicular blood vessels of adult and aged SHRs compared to Wistar rats. Thus, impaired sperm quality was observed in SHRs of different ages and was improved by sub-chronically blocking the alpha 1-adrenergic receptor. (AU)

• Processo FAPESP: 23/05115-2 - Efeito do tratamento com AP39 ou ATB346, doadores de H2S mitocondrial e híbrido, respectivamente, em modelo experimental de trombose: Ensaios in vivo em vasos sanguíneos cerebrais e in vitro
• Beneficiário: Stephen Fernandes de Paula Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 20/12616-0 - Efeito da idade e da hipertensão arterial sobre a qualidade dos espermatozoides e a microcirculação testicular de ratos
• Beneficiário: Nicolle Rakanidis Machado
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 20/07212-7 - Efeito do tratamento com doxiciclina, inibidor de protease inibitória secretada (SLPI), aldosterona inalada ou nanopartículas de ouro em modelo murino de síndrome do desconforto respiratório agudo
• Beneficiário: Stephen Fernandes de Paula Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Regular