Human brain tissue cultures: a unique ex vivo model to unravel the pathogenesis of neurotropic arboviruses

Autor(es)

Almeida, Glaucia M. ; Silva, Bruna M. ; Arruda, Eurico ; Sebollela, Adriano

• Tipo de documento: Artigo Científico
• Fonte: CURRENT OPINION IN VIROLOGY; v. 70, p. 8-pg., 2025-02-01.

Resumo

Arboviruses are transmitted by arthropods, and their spread from deforestation, climate change, and global mobility. Arbovirus infection in human results in symptoms ranging from mild to lifethreatening, with the impairment of central nervous system functions being reported in severe cases. Despite its clinical relevance, the mechanisms by which arboviruses led to neural dysfunction are still poorly understood. The lack of a widespread human central nervous system model to study the virus-host interaction challenges the advance of our knowledge on these mechanisms. In this context, human brain-derived ex vivo models have the advantage of preserving cellular diversity, cell connections, and tissue cytoarchitecture found in human brain, raising them as a powerful strategy to elucidate the cellularmolecular alterations underlying brain diseases. Here, we review recent advances in the field of neurotropic arboviruses obtained using ex vivo human brain tissue as the experimental model. (AU)

• Processo FAPESP: 21/10925-8 - Oligômeros neurotóxicos do peptídeo beta-amiloide: isolamento bioquímico, estudos conformacionais e neutralização em modelos da Doença de Alzheimer
• Beneficiário: Adriano Silva Sebollela
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/26119-0 - Vírus emergentes e reemergentes: biologia, patogênese e prospecção
• Beneficiário: Eurico de Arruda Neto
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 21/12263-2 - Alterações teciduais-moleculares associadas a infecções virais em culturas de fatias de cérebro humano adulto
• Beneficiário: Glaucia Maria de Almeida
• Modalidade de apoio: Bolsas no Brasil - Doutorado