| Texto completo | |
| Autor(es): |
Garcia, Maria Laura da Cruz
;
Paixao, Rafaela Ribeiro
;
Pazin, Wallance M.
;
Oliveira, Osvaldo N.
;
Cremer, Paul S.
;
Carlos, Rose Maria
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Langmuir; v. 40, n. 52, p. 11-pg., 2024-12-17. |
| Resumo | |
This study explores the interactions of the cis-[Ru(phen)2(Apy)2]2+ complex (RuApy, phen = 1,10-phenanthroline, Apy = 3,4-aminopyridine) with model lipid membranes to explain the role this complex plays in mitigating A beta toxicity in PC12 neuronal cells. Fluorescence quenching, surface pressure isotherms in Langmuir monolayers, and infrared reflection-absorption analyses revealed that the positively charged RuApy interacts with the phosphate headgroups of monolayers, indirectly affecting ester carbonyl groups through hydrogen bonding with the amino group of the pyridine ligand of RuApy. These results offer a scenario for the protective effect of RuApy against A beta toxicity in neuronal cells in which these interactions shield the electrostatic interactions of A beta with lipid membranes, preserving membrane integrity and mitigating the deleterious influence of A beta. This opens new avenues for antiamyloid strategies, focusing on compounds that prevent salt-bridge formation between bilayer membranes and amyloid proteins, aiding in the rational design of effective antiamyloid agents for therapeutic application. (AU) | |
| Processo FAPESP: | 22/03380-8 - Interações entre o peptídeo beta amiloide e modelos de membrana lipídica com o complexo cis-[Ru(phen)2(3,4Apy)2]2+ como sonda luminescente |
| Beneficiário: | Maria Laura da Cruz Garcia |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Mestrado |
| Processo FAPESP: | 21/02436-7 - Influência do tipo de lipídeo e peptídeo beta amiloide sobre a perturbação da membrana e consequências na toxicidade de células neuronais observadas pelas respostas luminescentes do complexo RuApy |
| Beneficiário: | Maria Laura da Cruz Garcia |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |