Dentin remineralization induced by experimental composites containing calcium orthophosphate particles

Autor(es)

Campos, Amanda Lopes ; Chiari, Marina Damasceno Souza de Carvalho ; Vela, Beatriz Fonseca ; Trinca, Rafael Bergamo ; Balbinot, Gabriela de Souza ; Collares, Fabricio Mezzomo ; Braga, Roberto Ruggiero

• Tipo de documento: Artigo Científico
• Fonte: Dental Materials; v. 41, n. 3, p. 7-pg., 2025-03-01.

Resumo

Objectives: This study aimed to verify if composites containing dicalcium phosphate dihydrate particles (DCPD) are able to induce dentin remineralization in vitro. Additionally, the mechanical properties of the materials were tested. Methods: Four composites with 50 vol% inorganic content and 1 BisGMA: 1 TEGDMA (mols) were prepared, with different DCPD:glass ratios (50:0, 40:10, 30:20 and 0:50). Ca2 + release in water was monitored for 8 weeks using inductively coupled plasma optical emission spectrometry (n = 3). Composites were applied to artificial lesions (180 mu m in depth) prepared in dentin discs and the specimens were kept in simulated body fluid for 8 weeks (n = 8-10). Dentin elastic modulus (EM) and hardness (H) across the lesion were determined by nanoindentation (5 mN, 5 s). Mineral density was determined by microCT. Composite degree of conversion (DC) was determined by near-FTIR spectroscopy (n = 3). Fracture strength and elastic modulus were determined using biaxial flexural test (n = 10). Data were analysed by ANOVA/Tukey test, except for mineral density (Kruskal-Wallis, alpha:0.05). Results: Ca2+ release increase linearly with DCPD fraction in the composite (p < 0.001). Lesions kept in contact with composites containing 40 % and 50 % DCPD presented significant increases in EM and H in the outer region (0-90 mu m) and in EM in the inner region (90-180 mu m) compared to the negative control. MicroCT was not able to differentiate among treatments. DCPD-containing composites presented DC higher than the control (p < 0.01). Flexural strength and modulus were inversely related to DCPD content (p < 0.001). Significance: The composite containing 40 vol% DCPD presented the best compromise between mechanical properties and remineralization potential. (AU)

• Processo FAPESP: 20/13983-6 - Efeito da temperatura, pH e concentração de precursores na síntese de partículas de DCPD e sua aplicação em um compósito resinoso remineralizante
• Beneficiário: Rafael Bergamo Trinca
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/04737-4 - Remineralização dentinária com o uso de um compósito contendo partículas de ortofosfato de cálcio associado a um precursor mineral em fase líquida (Polymer-Induced Liquid-Precursor, PILP): desenvolvimento de material, estudos in vitro e clínico aleatorizado
• Beneficiário: Roberto Ruggiero Braga
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 21/09657-9 - Remineralização dentinária funcional com o uso um análogo biomimético, associado ou não a um compósito contendo ortofosfato de cálcio
• Beneficiário: Amanda Lopes Campos
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto