| Texto completo | |
| Autor(es): |
de Lima, Lucas F.
;
Ferreira, Andre L.
;
Awasthi, Sita
;
Torres, Marcelo D. T.
;
Friedman, Harvey M.
;
Cohen, Gary H.
;
de Araujo, William R.
;
de la Fuente-Nunez, Cesar
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CELL REPORTS PHYSICAL SCIENCE; v. 4, n. 9, p. 17-pg., 2023-09-20. |
| Resumo | |
Herpes simplex virus type 2 (HSV-2) infection, which is almost exclusively sexually transmitted, causes genital herpes. Although this lifelong and incurable infection is extremely widespread, currently there is no readily available diagnostic device that accurately detects HSV-2 antigens to a satisfactory degree. Here, we report an ultrasensitive electrochemical device that detects HSV-2 antigens within 9 min and costs just $1 (USD) to manufacture. The electrochemical biosensor is biofunctionalized with the human cellular receptor nectin-1 and detects the glycoprotein gD2, which is present within the HSV-2 viral envelope. The performance of the device is tested in a guinea pig model that mimics human biofluids, yielding 88.9% sensitivity, 100.0% specificity, and 95.0% accuracy under these conditions, with a limit of detection of 0.019 fg mL-1 for gD2 protein and 0.057 PFU mL-1 for titered viral samples. Importantly, no cross -reactions with other viruses were detected, indicating the adequate robustness and selectivity of the sensor. Our low-cost technology could facilitate more frequent testing for HSV-2. (AU) | |
| Processo FAPESP: | 22/03250-7 - Desenvolvimento de biossensores portáteis e acessíveis para diagnósticos clínicos de infecções bacterianas e virais |
| Beneficiário: | William Reis de Araujo |
| Modalidade de apoio: | Auxílio à Pesquisa - Projeto Inicial |
| Processo FAPESP: | 18/08782-1 - Sensores químicos miniaturizados e integrados: novas plataformas de fabricação para aplicações biológicas, clínicas e ambientais |
| Beneficiário: | Mauro Bertotti |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |