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| Autor(es): Mostrar menos - |
Bertaglia, Raquel Santilone
[1]
;
Reissler, Joyce
[1]
;
Lopes, Francis Silva
[2]
;
Garrido Cavalcante, Walter Luiz
[3]
;
Carani, Fernanda Regina
[1]
;
Padovani, Carlos Roberto
[4]
;
Rodrigues, Sergio Augusto
[4]
;
Cigogna, Antonio Carlos
[5]
;
Carvalho, Robson Francisco
[1]
;
Henrique Fernandes, Ana Angelica
[6]
;
Gallacci, Marcia
[3]
;
Silva, Maeli Dal Pai
[1]
Número total de Autores: 12
|
| Afiliação do(s) autor(es): | [1] Sao Paulo State Univ, Dept Morphol, Inst Biosci, UNESP, BR-18618000 Botucatu, SP - Brazil
[2] Univ Oeste Paulista, Dept Physiotherapy, BR-19050920 Presidente Prudente, SP - Brazil
[3] Sao Paulo State Univ, Dept Phamacol, Inst Biosci, UNESP, BR-18618000 Botucatu, SP - Brazil
[4] Sao Paulo State Univ, Dept Bioestat, Inst Biosci, UNESP, BR-18618000 Botucatu, SP - Brazil
[5] Sao Paulo State Univ, Dept Internal Med, Sch Med, UNESP, BR-18618000 Botucatu, SP - Brazil
[6] Sao Paulo State Univ, Dept Chem & Biochem, Inst Biosci, UNESP, BR-18618000 Botucatu, SP - Brazil
Número total de Afiliações: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Molecular Histology; v. 42, n. 3, p. 205-215, JUN 2011. |
| Citações Web of Science: | 5 |
| Resumo | |
Heart failure (HF) is characterized by limited exercise tolerance, skeletal muscle atrophy, a shift toward fast muscle fiber, and myogenic regulatory factor (MRF) changes. Reactive oxygen species (ROS) also contribute to target organ damage in this syndrome. In this study, we investigated and compared morphofunctional characteristics and gene expression in Soleus (SOL-oxidative and slow twitching muscle) and in Extensor Digitorum Longus (EDL-glycolytic and fast twitching muscle) during HF. Two groups of rats were used: control (CT) and heart failure (HF), induced by a single injection of monocrotaline. MyoD and myogenin gene expression were determined by RT-qPCR, and MHC isoforms by SDS-PAGE; muscle fiber type frequency and cross sectional area (CSA) were analyzed by mATPase. A biochemical study was performed to determine lipid hydroperoxide (LH), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD); myography was used to determine amplitude, rise time, fall time, and fatigue resistance in both muscles. HF showed SOL and EDL muscle atrophy in all muscle fiber types; fiber frequency decreased in type IIC and muscle contraction fall time increased only in SOL muscle. Myogenin mRNA expression was lower in SOL and myoD decreased in HF EDL muscle. LH increased, and SOD and GSH-Px activity decreased only in HF SOL muscle. HF EDL muscle did not present changes in MHC distribution, contractile properties, HL concentration, and antioxidant enzyme activity. In conclusion, our results indicate that monocrotaline induced HF promoted more prominent biochemical, morphological and functional changes in SOL (oxidative and slow twitching muscle). Although further experiments are required to better determine the mechanisms involved in HF pathophysiology, our results contribute to understanding the muscle-specific changes that occur in this syndrome. (AU) | |
| Processo FAPESP: | 09/51060-8 - Avaliacao bioquimica, morfologica e funcional do musculo estriado esqueletico de ratos na insuficiencia cardiaca. |
| Beneficiário: | Joyce Reissler |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |