Chitosan-gelatin/hydroxyapatite-based scaffold associated with mesenchymal stem cells differentiate into osteoblasts improves the surface of the bone lesion in mice C57BL/6J

Autor(es)

Carvalho, Janicy Arantes ; Jayme, Cristiano Ceron ; Matsuo, Flavia Sayuri ; Tedesco, Antonio Claudio

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Polymer Science; v. 141, n. 8, p. 15-pg., 2023-11-28.

Resumo

Extensive injuries to bone tissue are still considered a significant clinical challenge; therefore, developing new bone tissue engineering (BTE) strategies is still necessary. This work aims to construct and characterize a chitosan-gelatin/hydroxyapatite-based (CG/H) scaffold to provide well-design support for mesenchymal stem cell (MSC) growth and differentiation to osteoblasts. First, the CG/H scaffolds are construct by freeze-drying. Then, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, x-ray diffraction, water uptake, and degradation profiles evaluate the material's surface. In addition, the CG/H morphological, biochemical, and MSC adhesion processes and growth behavior are also assess, indicating reasonable adhesion rates to the surface, low material cytotoxicity, and excellent alkaline phosphatase activity compared to control on the cellular framework. Based on these results, we obtain a highly biocompatible scaffold and that can support osteoblast differentiation. Finally, the in vivo studies demonstrate the CG/H scaffold with MSC adhere is capable of differentiating into osteoblasts, and the application of this scaffold is able to significantly enhance the closure of the bone lesion. Therefore, the CG/H scaffold has potential clinical application for bone regeneration. Developing new bone tissue engineering strategies is still necessary. This work aims to construct a chitosan-gelatin/hydroxyapatite-based (CG/H) scaffold to support mesenchymal stem cell (MSC). The CG/H scaffold presents reasonable adhesion rates, low cytotoxicity, and excellent alkaline phosphatase activity. The in vivo studies demonstrate the CG/H scaffold with MSC adhere can significantly enhance the closure of the bone lesion.image (AU)

• Processo FAPESP: 13/50181-1 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central
• Beneficiário: Antonio Claudio Tedesco
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 21/08111-2 - Materiais conversores de luz à base de terras raras: marcadores luminescentes, sensores e amplificadores ópticos
• Beneficiário: Hermi Felinto de Brito
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/10237-1 - Desenvolvimento e caracterização de scaffolds à base de PCL-PGE e DNA aplicados à engenharia de tecidos para regeneração óssea
• Beneficiário: Cristiano Ceron Jayme
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado