Assessment of products generated by photodynamic and sonodynamic action via Liquid Chromatography - Tandem Mass Spectrometry

Autor(es)

Ponce Ayala, Erika Toneth ; Barreiro, Juliana Cristina ; Silva e Carvalho, Iago ; Antunes, Camila Aparecida ; Alves, Fernanda ; Pratavieira, Sebastiao

• Tipo de documento: Artigo Científico
• Fonte: 2024 SBFOTON INTERNATIONAL OPTICS AND PHOTONICS CONFERENCE, SBFOTON IOPC 2024; v. N/A, p. 3-pg., 2024-01-01.

Resumo

Photodynamic and sonodynamic action rely on the interaction of a sensitizing molecule with light and ultrasound, respectively, generating reactive oxygen species. These interactions also lead to the generation of other products, which might be cytotoxic or photoactive. Identifying them may help to understand the mechanisms involved in each action. So, detecting and characterizing the products become very important. This work aimed to identify the products generated by photodynamic and sonodynamic action and discuss the degradation mechanisms associated with sonodynamic action. The well-known organic sensitizers, Protoporphyrin IX, Methylene Blue, Curcumin, and Chlorin e6 were used to mediate photodynamic and sonodynamic action. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used as the detection method. The detected ions' mass/charge ratio and retention time were summarized. (AU)

• Processo FAPESP: 21/14446-7 - Equipamento multiusuário - EMU concedido no processo 13/07276-1: Espectrômetro de massas com analisador do tipo triplo quadrupolo e Sistema de cromatografia gasosa acoplado a espectrômetro de massas
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 14/50857-8 - INCT 2014 - de Óptica Básica e Aplicada às Ciências da Vida
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 23/14868-4 - Compreensão dos mecanismos básicos da interação do ultrassom com sensibilizadores e tecidos biológicos para o aperfeiçoamento de terapia sonodinâmica.
• Beneficiário: Sebastião Pratavieira
• Modalidade de apoio: Auxílio à Pesquisa - Regular