Myenteric Denervation Downregulates Galectin-1 and-3 Expression in Gastric Carcinogenesis

Autor(es)

Estofolete, Cassia F. ^[1] ; Zucoloto, Sergio ^[2] ; Oliani, Sonia M. ^[3] ; Polli-Lopes, Ana Claudia ^[1] ; Gil, Cristiane D. ^{[4, 1]}

Afiliação do(s) autor(es)

^[1] Sao Jose do Rio Preto Sch Med FAMERP, Dept Anat, BR-15090000 Sao Paulo - Brazil

^[2] Ribeirao Preto Sch Med FMRP USP, Dept Pathol, BR-14049900 Sao Paulo - Brazil

^[3] Univ Estadual Paulista, IBILCE, Dept Biol, BR-15054000 Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Dept Morphol & Genet, BR-04023900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Digestive Diseases and Sciences; v. 56, n. 6, p. 1637-1644, JUN 2011.
• Citações Web of Science: 3

Resumo

Background This study evaluated the galectin-1 and -3 expression during N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced gastric carcinogenesis in denervated rat stomachs using benzalkonium chloride. Method Four experimental situations were evaluated: nondenervated and denervated stomachs without lesions and nondenervated and denervated stomachs with lesions. Sections of the pyloric region were stained with toluidine blue and incubated with mouse monoclonal anti-Gal-1 and rabbit polyclonal anti-Gal-3 for histopathological and immunohistochemical analysis, respectively. Result MNNG caused the development of benign and malignant epithelial lesions, which were more pronounced in nondenervated stomachs with lesions and accompanied by inflammatory cell-enriched stroma. By immunostaining, the epithelial cells, blood vessels, muscle layer, and myenteric plexus were Gal-1 and -3 positive. Gal-3 was also detected in the gastric crypts, mucus secretion, and fibroblasts of pyloric fragments. Development of lesions in denervated stomachs was associated with a significant decrease in Gal-1 and -3 expression in epithelial cells, mast cells, and neutrophil cytoplasm, compared with that of nondenervated stomach lesions (P < 0.01; P < 0.001; P < 0.001, respectively). Conclusion These results demonstrate that myenteric denervation downregulates endogenous Gal-1 and -3 expression, which might inhibit tumor development in this experimental model. (AU)

• Processo FAPESP: 08/05722-6 - Estudo da expressão das proteínas galectina-3 e anexina-A1 nas células inflamatórias em modelo experimental de tumorigênese gástrica em ratos
• Beneficiário: Cássia Fernanda Estofolete
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica