First Report of Rattail Cactus Necrosis-Associated Virus (Tobamovirus muricaudae) in Symptomless Opuntia leucotricha in Brazil

Autor(es)

Guimaraes, Jandson Jose do Vale ; Del Corona, Fernanda de Padua ; Bello, Vinicius Henrique ; Ramos-Gonzalez, Pedro Luis ; Lorenzi, Harri ; Kitajima, Elliot Watanabe

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHYTOPATHOLOGY; v. 173, n. 2, p. 3-pg., 2025-03-01.

Resumo

In 2024, a tobamovirus was found in a symptomless prickly pear (Opuntia leucotricha) plant in Brazil. Transmission electron microscopy examinations revealed tobamovirus-like particles ca. 300 nm long in negatively stained cladode's extracts from an asymptomatic O. leucotricha plant. High-throughput sequencing resulted in a 6374 nucleotide contig that shares 98.68% nucleotide sequence identity with rattail cactus necrosis-associated virus (RCNaV, Tobamovirus muricaudae), first detected in Aporocactus flagelliform, in South Korea. The near-complete genome of the RCNaV_BR1 isolate has the canonical genomic organisation of viruses in the genus Tobamovirus. The viral infection in the cactus was validated by RT-PCR using RCNaV_BR1-specific primers followed by Sanger sequencing. The RCNaV_BR1 could be mechanically transmitted to the experimental hosts Chenopodium amaranticolor, C. quinoa and Datura stramonium, and by grafting to some cactus plants (dragon fruit-Selenicereus spp., Opuntia brasiliensis, Epiphyllum sp.), which remained symptomless. This is the first report of RCNaV infecting any cactaceae in Brazil. (AU)

• Processo FAPESP: 24/06440-7 - Levantamento de vírus e viróides de plantas no Brasil, com ênfase naqueles infectando em cactáceas e prosseguimento na organização da lista de vírus e viróides descritos no Brasil após 2018. Fse IV
• Beneficiário: Elliot Watanabe Kitajima
• Modalidade de apoio: Auxílio à Pesquisa - Regular