| Texto completo | |
| Autor(es): Mostrar menos - |
Ripari, Nicolas
;
Lopes, Emilly Camargo
;
Francisco, Aleff Ferreira
;
de Almeida, Jonatas Felipe Santos
;
Honorio, Mariana da Silva
;
Fernandes Junior, Ary
;
Fontes, Marcos Roberto de Mattos
;
Tanimoto, Matheus Hikaru
;
Calderon, Leonardo de Azevedo
;
Bastos, Jairo Kenupp
;
Sforcin, Jose Mauricio
Número total de Autores: 11
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Phytomedicine; v. 140, p. 10-pg., 2025-03-09. |
| Resumo | |
Background: Propolis has been traditionally used to treat inflammatory and infectious diseases, and it is still used and researched worldwide. Methicillin-resistant Staphylococcus aureus (MRSA) may cause invasive infections and propolis anti-MRSA activity has been analyzed. Purpose: A standardized red propolis extract (SRPE), its benzophenones-rich fraction (BRF), and isolated benzophenones (guttiferone E - GUT E, and oblongifolin B - OBL B) were assayed for their antibacterial and immunomodulatory action. Methods: Formulations (BRP28, BRP29, BRP150, BRP153, and BRPLUS) were prepared and their minimum inhibitory concentrations (MIC) were assessed. The synergistic action of GUT E with antimicrobials was evaluated on a wound-isolated MRSA, as well as the inhibition of biofilm formation by the formulations (BRP28 and BRP29) and GUT E. Tohoku Hospital Pediatrics-1 (THP-1) cells were used to investigate cytokine production and the bactericidal activity of suppressed macrophages against MRSA. Computational predictions were performed with GUT E and antimicrobials to observe their interaction with the active and allosteric site of penicillin-binding protein 2a (PBP2a). Results: SRPE and BRF were not efficient against MRSA while GUT E and OBL B exerted a potent activity. GUT E exerted a synergistic effect with carbapenems and vancomycin. BRP28, BRP29, and GUT E inhibited biofilm formation and increased the antibacterial capacity of suppressed macrophages, with no differences in cytokine production. GUT E showed a high binding affinity to PBP2a. Conclusion: GUT E exhibited a direct anti-MRSA activity and indirectly enhanced the macrophage bactericidal activity. Molecular docking suggested that GUT E has a versatile interaction with PBP2a. (AU) | |
| Processo FAPESP: | 23/01436-9 - Ação antibacteriana, imunomoduladora e cicatrizante do extrato padronizado de própolis vermelha e da fração rica em benzofenonas: predições computacionais e desenvolvimento de produto tópico para tratamento de infecções cutâneas |
| Beneficiário: | Nicolas Ripari |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 17/04138-8 - Realização de estudos químicos, analíticos, biológicos, farmacológicos e tecnológicos para preenchimento das lacunas no desenvolvimento do setor de própolis brasileiro |
| Beneficiário: | Jairo Kenupp Bastos |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |