| Texto completo | |
| Autor(es): Mostrar menos - |
Rodrigues, Bruno Mangili de Paula
;
Hernandes, Paloma Molina
;
Balvedi, Rafael Canalle
;
Alves, Higor Ferreira Martins
;
da Silva, Karoline Paiva
;
Bicudo, Rogrio de Campos
;
Eberhardt, Marcelo Jung
;
Poletto, Fernanda
;
Paese, Karina
;
Guterres, Silvia Staniscaski
;
Khan, Asmat Ullah
;
Pohlmann, Adriana Raffin
;
Ferrarini, Stela Regina
;
Cysne, Norberto
Número total de Autores: 14
|
| Tipo de documento: | Artigo Científico |
| Fonte: | International Journal of Pharmaceutics; v. 679, p. 19-pg., 2025-06-30. |
| Resumo | |
The burgeoning interest in cannabinoid-based therapies for mental disorders motivates research into their efficacy. This study investigates the acute effects of nanoencapsulated Cannabidiol (CBD) on panic attack-like defensive responses elicited in mice by the venomous snake Bothrops jararaca in polygonal arenas validated as a panic attack experimental model. The aim of this work was to investigate if polymeric lipid corenanoencapsulated CBD at low doses was able to cause antiaversive effects in comparison to non-encapsulated CBD at a higher dose. Mice were habituated in the enriched polygonal arena during three days and treated with either CBD in a dose already demonstrated to cause antiaversive effects (3 mg/kg), used as a positive control, or nanostructured CBD at much lower doses before snake confrontation. Both non-encapsulated and nanoencapsulated CBD significantly attenuated antipredatory responses in mice. Interestingly. Polymeric lipid core-nanoencapsulated CBD, particularly at lower doses, attenuated panic-like responses, including defensive attention, flat back approach, and escape behaviours to safe places after reaching limbic system structures. Additionally, rhodamine-labeled polymeric lipid core-nanoencapsulated CBD, administered either intraperitoneally or intranasally, effectively crossed the blood-brain barrier, with fluorescence observed in multiple limbic and paralimbic brain structures implicated in the elaboration of defensive/antipredatory behavioural responses. These findings indicate that nanoencapsulation improves the bioavailability of CBD and its targeting of the central nervous system, thereby supporting its potential for low-dose therapeutic applications in the treatment of anxiety and panic disorders. (AU) | |
| Processo FAPESP: | 12/03798-0 - Envolvimento de receptores opióides e endocanabinóides da substância negra na modulação da atividade da via GABAérgica nigro-tectal durante a organização do comportamento de defesa eliciado por roedores confrontados com serpentes veneníferas |
| Beneficiário: | Norberto Cysne Coimbra |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 17/11855-8 - Estudo da influência de endocanabinoides do corpo estriado e do mesencéfalo ventral na atividade das projeções GABAérgicas neoestriado-nigro-tectais durante as reações de pânico de roedores ameaçados por serpentes peçonhentas |
| Beneficiário: | Norberto Cysne Coimbra |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 24/01368-6 - Explorando o papel do sistema orexinérgico e sua interação com os receptores canabinóides na substância cinzenta periaquedutal dorsal: implicações para a modulação de respostas defensivas associadas ao pânico. |
| Beneficiário: | Paloma Molina Hernandes |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 20/15050-7 - Estudo do papel da sinalização endocanabinoide e endovaniloide no paleoestriado, na atividade do duplo elo conectivo GABAérgico neoestriado-pálido-nigro-tectal durante a organização do medo instintivo de animais confrontados com serpentes |
| Beneficiário: | Norberto Cysne Coimbra |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |