Direct injection of native aqueous matrices by achiral-chiral chromatography ion trap mass spectrometry for simultaneous quantification of pantoprazole and lansoprazole enantiomers fractions

Autor(es)

Barreiro, Juliana Cristina ^[1] ; Vanzolini, Kenia Lourenco ^[1] ; Cass, Quezia Bezerra ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Chem, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Chromatography A; v. 1218, n. 20, p. 2865-2870, MAY 20 2011.
• Citações Web of Science: 38

Resumo

A two-dimensional liquid chromatography system coupled to ion-trap tandem mass spectrometer (2DLC-IT-MS/MS) was employed for the simultaneous quantification of pantoprazole and lansoprazole enantiomers fractions. A restricted access media of bovine serum albumin octyl column (RAM-BSA C(8)) was used in the first dimension for the exclusion of the humic substances, while a polysaccharide-based chiral column was used in the second dimension for the enantioseparation of both pharmaceuticals. The results described here show good selectivity, extraction efficiency, accuracy, and precision with detection limits of 0.200 and 0.150 mu g L(-1) for the enatiomers of pantoprazole and lansoprazole respectively, while employing a small amount (1.0 mL) of native water sample per injection. This work reports an innovative assay for monitoring work, studies of biotic and abiotic enantioselective degradation and temporal changes of enantiomeric fractions. (C) 2011 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 06/05126-9 - Determinação enantiomérica dos fármacos: omeprazol, lansoprazol e pantoprazol em águas residuárias urbanas da cidade de São Carlos, SP
• Beneficiário: Kenia Lourenço Vanzolini
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 08/05778-1 - Fármacos quirais em águas residuais: quantificação e estudos de mecanismos de separação enantiosseletiva
• Beneficiário: Juliana Cristina Barreiro
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado