Polymerized laminin-modified microcapsules improve pancreatic islet resilience towards cytokine induced inflammatory stress and lower chemoattractant cytokine secretion

Autor(es)

Silva, Isaura Beatriz Borges ; Borghuis, Theo ; Qin, Tian ; Sogayar, Mari Cleide ; de Vos, Paul

• Tipo de documento: Artigo Científico
• Fonte: MATERIALS TODAY BIO; v. 32, p. 20-pg., 2025-05-09.

Resumo

Encapsulation of human pancreatic islets in semipermeable membranes offers a promising, less invasive alternative to whole pancreas transplantation for Type 1 Diabetes (T1D), potentially reducing the need for immunosuppressive drugs and improving graft survival. However, the inflammatory environment during transplantation poses significant challenges, often leading to substantial graft loss. This study explores modifying the intracapsular environment with extracellular matrix (ECM) molecules - chondroitin sulfate (ChS), laminin (LN), and polymerized laminin (pLN) - to enhance islet cell resilience against cytokine-induced stress, associated with the post-transplantation environment. Encapsulated mouse insulinoma n-cells (MIN6) and human islets were exposed to pro-inflammatory cytokines (IL-1n, IFN-gamma, TNF-alpha) over 1, 3, and 5 days. The results showed that LN and pLN in combination with ChS particularly improved outcomes, with LN reducing oxidative stress and pLN significantly lowering cell death and pro-inflammatory chemokine MCP-1 production. These findings highlight the potential of ECM-modified encapsulation to enhance the survival of transplanted islets, offering a more favorable approach for T1D treatment and allowing transplantation of pancreatic islets with fewer islets. (AU)

• Processo FAPESP: 21/12497-3 - Avaliação de um polímero de pectina e da prolactina recombinante humana na melhoria da viabilidade e funcionalidade de ilhotas pancreáticas microencapsuladas
• Beneficiário: Isaura Beatriz Borges Silva
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 19/21935-4 - Caracterização do transcritoma de células-tronco embrionárias durante sua diferenciação em células produtoras de insulina na ausência e presença de fatores peptídicos de crescimento/diferenciação
• Beneficiário: Isaura Beatriz Borges Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/05311-2 - Medicina regenerativa visando à terapia de doenças crônico-degenerativas (câncer e diabetes)
• Beneficiário: Mari Cleide Sogayar
• Modalidade de apoio: Auxílio à Pesquisa - Temático