Host-parasite-environment relationship, morphology and molecular analyses of Henneguya eirasi n. sp parasite of two wild Pseudoplatystoma spp. in Pantanal Wetland, Brazil

Autor(es)

Naldoni, J. ^[1] ; Arana, S. ^[2] ; Maia, A. A. M. ^[3] ; Silva, M. R. M. ^[3] ; Carriero, M. M. ^[3] ; Ceccarelli, P. S. ^[4] ; Tavares, L. E. R. ^[5] ; Adriano, E. A. ^[6]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Univ Estadual Campinas, Dept Parasitol, BR-13083970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Univ Estadual Campinas, Dept Histol & Embriol, BR-13083970 Campinas, SP - Brazil

^[3] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Dept Ciencias Basicas, BR-13635900 Pirassununga, SP - Brazil

^[4] Inst Chico Mendes Conservacao Biodiversidade ICMb, Ctr Nacl Pesquisa & Conservacao Peixes Continenta, BR-13630970 Pirassununga, SP - Brazil

^[5] Univ Fed Mato Grosso Sul UFMS, Ctr Ciencias Biol & Saude, Dept Patol, BR-79070900 Campo Grande, MS - Brazil

^[6] Univ Fed Sao Paulo, Dept Ciencias Biol, BR-09972270 Diadema, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Veterinary Parasitology; v. 177, n. 3-4, p. 247-255, MAY 11 2011.
• Citações Web of Science: 25

Resumo

A new myxosporean species, Henneguya eirasi n. sp., is described parasitizing the gill filaments of Pseudoplatystoma corruscans and Pseudoplatystoma fasciatum (Siluriformes: Pimelodidae) caught in the Patanal Wetland of the state of Mato Grosso, Brazil. The parasite formed white, elongated plasmodia measuring up to 3 mm. Mature spores were ellipsoidal in the frontal view, measuring 37.1 +/- 1.8 mu m in total length, 12.9 +/- 0.8 mu m in body length, 3.4 +/- 0.3 mu m in width, 3.1 +/- 0.1 mu m in thickness and 24.6 +/- 2.2 mu m in the caudal process. Polar capsules were elongated and equal in size, measuring 5.4 +/- 0.5 mu m in length and 0.7 +/- 0.1 mu m in width. Polar filaments had 12-13 coils. Histopathological analysis revealed that the parasite developed in the sub-epithelial connective tissue of the gill filaments and the plasmodia were surrounded by a capsule of host connective tissue. The plasmodia caused slight compression of the adjacent tissues, but no inflammatory response was observed in the infection site. Ultrastructure analysis revealed a single plasmodial wall connected to the ectoplasmic zone through numerous pinocytotic canals. The plasmodial wall exhibited numerous projections and slightly electron-dense material was found in the ectoplasm next to the plasmodial wall, forming a line just below the wall. Partial sequencing of the 18S rDNA gene of H. eirasi n. sp. obtained from P. fasciatum resulted in a total of 1066 bp and this sequence did not match any of the Myxozoa available in the GenBank. Phylogenetic analysis revealed the Henneguya species clustering into clades following the order and family of the host fishes. H. eirasi n. sp. clustered alone in one clade, which was the basal unit for the clade composed of Henneguya species parasites of siluriform ictalurids. The prevalence of the parasite was 17.1% in both fish species examined. Parasite prevalence was not influenced by season, host sex or host size. (C) 2011 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 06/59075-6 - Mixosporídeos parasitos de Pseudoplatystoma corruscans (pintado), Pseudoplatystoma fasciatum (cachara), Salminus brasiliensis (dourado) e Brycon hilari (piraputanga) oriundos de ambiente natural e de sistemas de criação: taxonomia e interação parasito-hospedeiro
• Beneficiário: Edson Aparecido Adriano
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores