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Effect of titanium dioxide nanotubes incorporated into conventional glass ionomer cement on L. acidophilus

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Sena, Layse de Gois ; Meyer, Maria Davoli ; Ricardo, Mariana Gallante ; Rontani, Julia Puppin ; Araujo, Isaac Jordao de Souza ; Pecorari, Vanessa Arias ; Martinez, Elizabeth Ferreira ; Teixeira, Lucas Novaes ; Nociti-Junior, Francisco Humberto ; Lisboa-Filho, Paulo Noronha ; Kantovitz, Kamila Rosamilia
Número total de Autores: 11
Tipo de documento: Artigo Científico
Fonte: BRAZILIAN ORAL RESEARCH; v. 39, p. 10-pg., 2025-01-01.
Resumo

The present in vitro study evaluated the effect of titanium dioxide nanotubes (nTiO(2)) incorporated into glass ionomer cement (GIC) on the growth and viability of Lactobacillus acidophilus (L. acidophilus). GIC (Ketac Molar EasyMix (R) = KM) was added to concentrations of 0%, 3%, 5%, 7% by weight of nTiO(2) (20 nm in size). L. acidophilus strains (1x10(8) CFU/mL) were cultivated on GIC discs with or without the addition of nTiO(2) for 1, 3 and 7 days, and the following parameters were evaluated: inhibition zone (mm) (n = 6); cell viability (Live/Dead) (n = 6); cell morphology (SEM) (15 KV, 2000X, n = 3). The data were submitted to ANOVA and the Tukey and Dunnett tests (alpha = 0.05). Regarding the agar diffusion test, there was no difference between GIC and the groups containing nTiO(2) (p > 0.05). As for bacterial viability, the percentage of viable bacteria was lower for GIC+7% nTiO(2) (p <= 0.05). There was no difference in the percentage of non-viable bacteria (p > 0.05). In addition, the morphology of L. acidophilus did not change in the presence of nTiO(2). It can be concluded that the incorporation of titanium dioxide nanotubes into GIC, particularly at 5%, reduced L. acidophilus viability, and might hence interfere negatively with the initial colonization process of the bacterial biofilm. (AU)

Processo FAPESP: 16/13786-0 - Influência de Nova Nanotecnologia nas Propriedades Físico-Químicas-Biológicas do Cimento de Ionômero de Vidro Convencional
Beneficiário:Kamila Rosamilia Kantovitz
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 19/14078-8 - Avaliação do efeito da incorporação de nanotubos de dióxido de titânio ao cimento de ionômero de vidro convencional sobre suas propriedades biológicas
Beneficiário:Kamila Rosamilia Kantovitz
Modalidade de apoio: Auxílio à Pesquisa - Regular