| Texto completo | |
| Autor(es): |
dos Santos, Sidney S.
;
Cerqueira, Miguel Angelo
;
Azevedo, Ana Gabriela
;
Pastrana, Lorenzo M.
;
Aouada, Fauze Ahmad
;
Tanaka, Fabricio C.
;
Perotti, Gustavo Frigi
;
de Moura, Marcia Regina
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PHARMACEUTICALS; v. 18, n. 6, p. 17-pg., 2025-05-26. |
| Resumo | |
Background/Objectives: The rise of bacterial resistance and the search for alternative, biocompatible antimicrobial materials have driven interest in natural-based nanocomposites. In this context, silver nanoparticles (AgNPs) have shown broad-spectrum antibacterial activity, and bacterial cellulose (BC) is widely recognized for its high purity, hydrophilicity, and biocompatibility. This study aimed to develop a bio-based BC-AgNP nanocomposite via green synthesis using Astrocaryum aculeatum (tucum & atilde;) extract and assess its antimicrobial performance for wound dressing applications. Methods: BC was biosynthesized via green tea fermentation (20 g/L tea and 100 g/L sugar) and purified prior to use. AgNPs were obtained by reacting aqueous tucum & atilde; extract with silver nitrate (0.1 mmol/L) at pH (9) and temperature (40 degrees C). BC membranes were immersed in the AgNPs dispersion for 7 days to form the nanocomposite. Characterization was performed using UV-Vis, DLS, TEM, SEM-EDS, FTIR, XRD, ICP-OES, and swelling analysis. Antibacterial activity was evaluated using the disk diffusion method against Staphylococcus aureus and Escherichia coli (ATCC 6538 and 4388). Results: The UV-Vis spectra revealed a gradual decrease in the surface plasmon resonance (SPR) band over 7 days of incubation with BC, indicating progressive incorporation of AgNPs into the membrane. ICP analysis confirmed silver incorporation in the BC membrane at 0.00215 mg/mL, corresponding to 15.5% of the initial silver content. Antimicrobial assays showed inhibition zones of 6.5 +/- 0.5 mm for S. aureus and 4.3 +/- 0.3 mm for E. coli. Conclusions: These findings validate the successful formation and antimicrobial performance of the BC-AgNP nanocomposite, supporting its potential use in wound care applications. (AU) | |
| Processo FAPESP: | 13/03643-0 - Estudo de novos veículos biodegradáveis carreadores de fertilizantes e defensivos agrícolas baseados em hidrogéis e silicatos nanoestruturados como uma alternativa no combate de impactos ambientais |
| Beneficiário: | Fauze Ahmad Aouada |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 13/07296-2 - CDMF - Centro de Desenvolvimento de Materiais Funcionais |
| Beneficiário: | Elson Longo da Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 18/18697-1 - Desenvolvimento de novos nanocompósitos magnéticos baseados em hidrogéis para avaliação como veículos carreadores de fármacos e insumos agrícolas |
| Beneficiário: | Fauze Ahmad Aouada |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |