Autor(es)

Ribeiro, Giulia M. ; Lahr, Daniel J. G.

• Tipo de documento: Artigo Científico
• Fonte: ACTA PROTOZOOLOGICA; v. 63, p. 9-pg., 2024-01-01.

Resumo

Unerstanding the physilogical and genetic diversity of single-cell eukaryotes remains big challenge for scientists. Single-cell from the deciduous marsh grass produced organic matter in the sediment surface the mean abundance of active amoebae +/- standard error mics techologies have revolutionized our possibilities for many previously uncharacerized groups. Despite the great dvancement, there of the mean(SEM) was higher (3 07 +/- 0 99 x 104/ L N = 7) In warmer months (May to September) the abundance of amoebae was lower is still a substantial gap in our genoic knowledge about eukaryotes, with les than 1 % of species sequenced. This knowledge gp is even (1 35 +/- 029 x 104 / L, N = 10) A multivariate linear regression analysis wasperformed relating amoeba abundance to four major water more pronounced in some microbial eukaryote groups such as Arcellinids. Arcellinida is a group of bioindicator protists that inhabit mostly freshwater and soil environments. Currently, we don't have any genome availabl for the Arcellinida group, undermiing our capacity to where: A = active amoebae densty (x 104/L) T = temperature (oC) L = tide level (m) S = Secchi dsc deph (cm) and C = bacterial nunderstand their biology, metabolic capacities, and resistance mechanisms that makes them useful as bioindicators. Using transcriptomic data only, we made great progress in this group by elucidating, for example, thei systematics, sex, and metabolism. This manuscrip highecosystems further research is warranted on their role in food webs and the car bon biogeochemical cycle within heterotrophic est uarine lights the potential of Arcellinida as a model for genomic studies, particularly the Arcella uspiensis species. Through comparative analysis, we demonstrate that despite its importance, transcriptomics hinders some limitations that need to be acknowledged. Finally, considering that w have much basic biology knowledge and protocols for Arcella uspnsis, we propose sequencing the genome of this species as a way to Ke y words:unlock furher potential insights into ecology, evolution, and function of Arcellinida. This manuscript emphasizes the importance of exploring genomics as a way to understand the ecology and evolution of microbial eukaryotic groups. (AU)

• Processo FAPESP: 19/22815-2 - Aplicação de Next Generation Long Read Sequencing à sistemática de protistas
• Beneficiário: Daniel José Galafasse Lahr
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/04585-3 - Decifrando as grandes tendências de evolução molecular e morfológica nos Amoebozoa
• Beneficiário: Daniel José Galafasse Lahr
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 19/22109-0 - Avaliação do impacto de condições ambientais na diversidade taxonômica e funcional de tecamebas
• Beneficiário: Giulia Magri Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado