| Texto completo | |
| Autor(es): |
Rigon, Roberta Balansin
;
Goncalez, Maira Lima
;
Severino, Patricia
;
Alves, Danilo Antonini
;
Santana, Maria H. A.
;
Souto, Eliana B.
;
Chorilli, Marius
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | COLLOIDS AND SURFACES B-BIOINTERFACES; v. 171, p. 5-pg., 2018-11-01. |
| Resumo | |
The present study focuses on the characterization of the cytotoxic profile on NIH3T3 mouse embryonic fibroblasts of solid lipid nanoparticles (SLN) optimized by a 2(2) full factorial design for skin administration. To build up the surface response charts, a design of experiments (DoE) based on 2 independent variables was used to obtain an optimized formulation. The effect of the composition of lipid and water phases on the mean particle size (z-AVE), polydispersity index (PdI) and zeta potential (ZP) was studied. The developed formulations were composed of 5.0% of lipid phase (stearic acid (SA), behenic alcohol (BA) or a blend of SA:BA (1:1)) and 4.7% of surfactants (soybean phosphatidylcholine and poloxamer 407). In vitro cytotoxicity using NIH3T3 fibroblasts was performed by MTT reduction assay. This factorial design study has proven to be a useful tool in optimizing SLN (z-AVE similar to 200 nm), which were shown to be non-cytotoxic. The present results highlight the benefit of applying statistical designs in the preparation and optimization of SLN formulations. (AU) | |
| Processo FAPESP: | 12/19568-4 - Avaliação do potencial de sistemas lipídicos nanoestruturados para administração cutânea de trans-resveratrol |
| Beneficiário: | Marlus Chorilli |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 13/21500-1 - Nanopartículas lipídicas sólidas para incorporação do trans-resveratrol no tratamento tópico do melanoma: avaliação in vitro e in vivo e elucidação dos mecanismos de internalização celular |
| Beneficiário: | Roberta Balansin Rigon |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 11/16888-5 - Desenvolvimento e caracterização de nanopartículas lipídicas sólidas para administração cutânea de trans-resveratrol |
| Beneficiário: | Roberta Balansin Rigon |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |