MMP1 and MMP20 contribute to tooth agenesis in humans

Autor(es):Mostrar menos -

Kuechler, Erika C. ^[1] ; Menezes, Renato ^[2] ; Callahan, Nicholas ^[2] ; Costa, Marcelo C. ; Modesto, Adriana ^[3] ; Meira, Raquel ^[4] ; Patir, Asli ^[5] ; Seymen, Figen ^[5] ; Paiva, Katiucia B. S. ^[6] ; Nunes, Fabio Daumas ; Granjeiro, Jose Mauro ^{[7, 8]} ; Vieira, Alexandre R. ^{[9, 3, 10, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Rio de Janeiro, Sch Dent, Dept Pediat Dent & Orthodont, Rio De Janeiro - Brazil

^[2] Univ Pittsburgh, Ctr Craniofacial & Dent Genet, Pittsburgh, PA 15261 - USA

^[3] Univ Pittsburgh, Sch Dent Med, Dept Pediat Dent, Pittsburgh, PA 15261 - USA

^[4] Brazilian Lutheran Univ, Dept Pediat Dent, Canons, RS - Brazil

^[5] Istanbul Univ, Dept Pedodont, Istanbul - Turkey

^[6] Univ Sao Paulo, Dept Oral Pathol, BR-05508 Sao Paulo - Brazil

^[7] Univ Fed Fluminense, Inst Biol, Dept Cellular & Mol Biol, Niteroi, RJ - Brazil

^[8] Univ Fed Fluminense, Unit Clin Res, Cell Therapy Ctr, Niteroi, RJ - Brazil

^[9] Univ Pittsburgh, Grad Sch Publ Hlth, Dept Human Genet, Pittsburgh, PA 15261 - USA

^[10] Univ Pittsburgh, Sch Dent Med, Dept Oral Biol, Pittsburgh, PA 15261 - USA

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 56, n. 5, p. 506-511, MAY 2011.
• Citações Web of Science: 12

Resumo

Objective: Variations in genes that are critical for tooth formation may contribute to the tooth agenesis. MMPs are potential candidate genes for dental alterations based on the roles they play during embryogenesis. The aim of this study was to investigate the possible association between MMP1, MMP3, and MMP20 and tooth agenesis. Methods: One hundred sixty-seven nuclear families from two different populations were analysed, 116 from Brazil and 51 from Turkey. Probands had at least one congenitally missing tooth. DNA samples were obtained from blood or saliva samples and genotyping was performed using TagMan chemistry. In addition, Mmp20 was selected for quantitative real-time polymerase chain reaction analysis with SYBR Green I Dye in mouse tooth development. Results: Associations between tooth agenesis and MMP1 (p = 0.007), and MMP20 (p = 0.03) were found in Brazilian families. In the total dataset, MMP20 continued to be associated with tooth agenesis (p = 0.01). Mmp20 was not expressed during the initial stages of tooth development. Conclusion: Our findings provide evidence that MMP1 and MMP20 play a role in human tooth agenesis. (C) 2010 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 07/04148-1 - Avaliação temporal e espacial da expressão dos genes (Ras, Myc, Fos, Jun e Msx) e seus respectivos fatores de transcrição regulados pelas metaloproteinases de matriz, seus inibidores teciduais e RECK durante a ossificação endocondral em camundongos
• Beneficiário: Fabio Daumas Nunes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/09274-8 - Avaliação temporal e espacial da expressão das metaloproteinases de matriz tipo de membrana (MT2, MT3, MT4, MT5 e MT6-MMP) durante a ossificação endocondral em camundongos
• Beneficiário: Fabio Daumas Nunes
• Modalidade de apoio: Auxílio à Pesquisa - Regular