| Texto completo | |
| Autor(es): |
de Souza-Neto, Reinaldo Rodrigues
;
Cavalcante, Lidia Nascimento
;
Carvalho, Isis Gabriela Barbosa
;
Curtolo, Maiara
;
Benedetti, Celso Eduardo
;
Takita, Marco Aurelio
;
Wang, Nian
;
de Souza, Alessandra Alves
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | MOLECULAR PLANT-MICROBE INTERACTIONS; v. 38, n. 4, p. 10-pg., 2025-08-07. |
| Resumo | |
The Citrus sinensis LATERAL ORGAN BOUNDERIES 1 (CsLOB1) gene, which is directly induced by the Xanthomonas citri subsp. citri effector PthA4, functions as a transcription factor and citrus canker susceptibility (S) gene. Genome editing of CsLOB1 has been shown to confer resistance to citrus canker disease. Previous studies revealed that the citrus CsLOB1-INDUCED EXPANSIN 1 gene (CsLIEXP1) is highly and directly upregulated by CsLOB1 in Xanthomonas citri subsp. citri-infected plants. Because expansins are associated with cell wall loosening, potentially facilitating bacterial colonization, the CsLOB1-dependent activation of CsLIEXP1 is thought to contribute to canker symptoms and disease progression. Thus, CsLIEXP1 likely represents a critical canker susceptibility gene. In this study, we employed CRISPR/Cas9 to disrupt the function of CsLIEXP1 by modifying its corresponding coding region in Citrus sinensis cultivar 'Hamlin' and evaluated the postinfection responses of edited plants. DNA sequencing confirmed the edition of the CsLIEXP1-edited plant, which exhibited 26.47% of CsLIEXP1 edited sequences. Furthermore, CsLIEXP1 protein accumulation was reduced in CsLIEXP1-edited plants compared with the wild type when infected with X. citri. Leaves of edited plants inoculated with X. citri showed significantly fewer canker symptoms, with lesions limited to the site of bacterial inoculation and less pronounced cellular hypertrophy compared with control plants. Our results show that CsLIEXP1 is a citrus canker S gene that acts downstream of CsLOB1, thus providing new insights into plant-pathogen interactions.Copyright (c) 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license. (AU) | |
| Processo FAPESP: | 23/05118-1 - Potencial das plantas de citros geneticamente modificadas supexpressando o gene rpfF no controle de doenças bacterianas de citros. |
| Beneficiário: | Alessandra Alves de Souza |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 17/13885-1 - Uso de CRISPR/Cas9 na edição de genes de suscetibilidade de Citrus sinensis a Xanthomonas citri subsp. Citri |
| Beneficiário: | Reinaldo Rodrigues de Souza Neto |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 13/10957-0 - Interação Xylella fastidiosa-inseto vetor-planta hospedeira e abordagens para o controle da clorose variegada dos citros e cancro cítrico |
| Beneficiário: | Alessandra Alves de Souza |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 14/50880-0 - INCT 2014: de genômica comparativa e funcional e melhoramento assistido de citros |
| Beneficiário: | Marcos Antonio Machado |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 20/04795-1 - Transformação de variedades de laranja doce para estudo funcional e/ou edição de genes visando resistência a doenças bacterianas de citros |
| Beneficiário: | Isis Gabriela Barbosa Carvalho |
| Modalidade de apoio: | Bolsas no Brasil - Programa Capacitação - Treinamento Técnico |
| Processo FAPESP: | 18/21990-2 - Uso de CRISPR/Cas9 na edição de genes de suscetibilidade de Citrus sinensis a Xanthomonas citri subsp. citri |
| Beneficiário: | Reinaldo Rodrigues de Souza Neto |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |