Autor(es)

Vilela, Felipe Pinheiro ; Rodrigues, Dalia dos Prazeres ; Beiting, Daniel ; Falcao, Juliana Pfrimer

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Microbiology; v. 136, n. 8, p. 14-pg., 2025-08-01.

Resumo

Aims: Analyze the survival and transcriptional response of Salmonella Infantis (S. Infantis) against acid, oxidative, increased osmolarity, and thermal stresses. Methods and results: 25 strains isolated from human, food, and veterinary sources in Brazil between 2013 and 2018 were analyzed. 8 log(10) CFU ml(-1) inoculums were exposed for 10 min, 1 h, or 24 h to acid stress (HCl pH 2.6); oxidative stress (15 mmol l(-1) H2O2); NaCl 9%; refrigeration (4 degrees C); freezing (-20 degrees C); and heating (63/74 degrees C). Most strains (n = 22) survived the acid stress after 10 min and 1 h. Strains maintained high populations under oxidative stress (>87.7%) and NaCl 9% (111.4%). No strains survived heating, while refrigeration prevented multiplication (similar to 101%), and freezing reduced populations (<92.6%). Strain 1143/14 had its transcriptome sequenced under acid and oxidative stresses and NaCl 9%. 14 up-regulated and 38 down-regulated transcripts under acid stress, and 407 up-regulated and 143 down-regulated under oxidative stress were expressed, associated with biological processes of response and adaptation. No transcripts were differentially expressed with NaCl 9%. Conclusions: Salmonella Infantis survival against most stresses represents a challenge for its control. The efficacy of heating reinforced its importance to control Salmonella in food. While acid survival involved the expression of virulence and broad stress response genes, oxidative stress survival showed the expression of genes specific to the adaptation to this condition. (AU)

• Processo FAPESP: 21/07365-0 - Avaliação genômica, transcriptômica e fenotípica de aspectos da virulência de linhagens de Salmonella Infantis isoladas de fontes diversas no Brasil
• Beneficiário: Felipe Pinheiro Vilela
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 22/07013-0 - Análises do genoma, transcriptoma, construção de mutantes e caracterização fenotípica de linhagens de Campylobacter coli isoladas de diversas fontes durante 25 anos no Brasil
• Beneficiário: Juliana Pfrimer Falcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 23/11427-7 - Análise transcriptômica de Salmonella infantis do Brasil sob condições de sobrevivência relacionadas a saúde humana e segurança alimentar
• Beneficiário: Felipe Pinheiro Vilela
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado