| Texto completo | |
| Autor(es): |
Perli, Gabriel
;
Braga, Carolyne B.
;
Bertuzzi, Diego L.
;
Soares, Marco C. P.
;
Ramos, Miguel
;
Es, Ismail
;
Ornelas, Catia
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | MATERIALS TODAY CHEMISTRY; v. 47, p. 10-pg., 2025-07-07. |
| Resumo | |
The development of innovative nanomaterials for antimicrobial applications has become increasingly important in the context of rising multidrug-resistant (MDR) infections. Silver nanoparticles (AgNPs) are potent antimicrobial agents due to their unique physicochemical properties and broad-spectrum bactericidal activity. However, the synthesis of ultra-small and monodisperse AgNPs with controlled size, shape, and stability remains a significant challenge. In this study, we present a novel reactive nanoemulsion approach that leverages the dual functionality of formamide as both an emulsifying solvent and a reducing agent, allowing rapid in situ reduction of Ag+ to Ag0 and the formation of AgNPs ranging from 2.5 to 8.0 nm (mean 4.8 +/- 1.0 nm) with low polydispersity. The Ac-Dex polymer network ensured particle stability and enabled co-encapsulation of doxycycline (DOX), producing a dual antimicrobial formulation (Ac-Dex-Ag-DOX NPs). These nanoparticles showed minimal cytotoxicity against MCF-7 cells and exhibited strong antibacterial activity against E. coli, with a minimal inhibitory concentration of 3.0 mu g mL-1 (calculated based on Ag concentration). The resultant hybrid material constitutes a hierarchical nano-in-nano architecture, a class of hybrid nanomaterials in which a primary functional nanoparticle (ultra-small AgNPs) is nested within a secondary nanoscale carrier (Ac-Dex NPs). This organization confers multilevel stabilization, precise size control, and dual antimicrobial functionality. The unprecedented combination of reactive nanoemulsion synthesis and polymer-mediated stabilization holds great potential for developing next-generation antimicrobial nanomaterials. (AU) | |
| Processo FAPESP: | 17/06146-8 - Desenvolvimento de nanocarregadores baseados em dendrímeros e nanopartículas poliméricas para a vetorização seletiva da goniotalamina, piplartina e monastrol |
| Beneficiário: | Carolyne Brustolin Braga |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 17/24488-3 - Desenvolvimento de Carregadores Nanoestruturados Baseados em Polisacarídeos para Entrega Controlada de Antibióticos e Nanopartículas de Prata |
| Beneficiário: | Gabriel Perli |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |