Polystyrene nanoplastic co-exposed to BPA and BPS induces cytotoxicity, genotoxicity, and alters ROS production in HepG2 cells

Autor(es)

Rocha, Cecilia Cristina de Souza ; Sanchez, Caroline Andolfato ; Souza, Marilia Cristina Oliveira ; Treviso, Estefani Maria ; Savietto, Gabriel Henrique ; Devoz, Paula Picoli ; de Freitas, Lucas Victor Pereira ; Antunes, Lusania Maria Greggi ; Paschoal, Jonas Augusto Rizzato ; Barbosa Junior, Fernando

• Tipo de documento: Artigo Científico
• Fonte: TOXICOLOGY IN VITRO; v. 109, p. 15-pg., 2025-12-01.

Resumo

During plastic degradation, it is fragmented into micro and nanoplastic, which can adsorb contaminants from the environment, increasing the plastics<acute accent>toxicity. Bisphenol A is used in plastic production and can be an endocrine disruptor. Bisphenol S is an analog of bisphenol A and has been used as an alternative to "BPA-free" products. Therefore, this is the first research proposing to verify whether nanoplastics associated with bisphenol A or S can increase toxicity in HepG2 cells. Nanoplastics associated with bisphenols could alter the cell viability of HepG2 cells compared to the group treated with the nanoplastic alone and concerning the respective bisphenol. The coexposure of nanoplastic to bisphenols A or S promoted cytotoxic and genotoxic damage in HepG2 cells, altering the reactive oxygen species production, increasing DNA strand breaks, and increasing apoptotic cells. Bisphenols A and S also showed cytotoxic and genotoxic effects in HepG2 cells. However, 8-OHdG was only detected in the group treated with the nanoplastic at the lowest concentration. This study highlights the cytotoxicity and genotoxicity of nanoplastics and bisphenols A and S, providing new insights into hepatocyte toxicity. (AU)

• Processo FAPESP: 18/24069-3 - Do biomonitoramento ao reconhecimento de assinaturas do exposoma humano visando antecipar riscos para uma saúde contínua
• Beneficiário: Fernando Barbosa Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 22/03476-5 - Avaliação da interação mercúrio-selênio em populações ribeirinhas da Amazônia combinando abordagens analíticas e toxicológicas de fronteira
• Beneficiário: Fernando Barbosa Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Regular