OPTIMIZATION OF CLAVULANIC ACID PRODUCTION BY STREPTOMYCES DAUFPE 3060 BY RESPONSE SURFACE METHODOLOGY

Autor(es)

Viana Marques, Daniela A. ^[1] ; Carneiro Cunha, Marcia N. ^[2] ; Araujo, Janete M. ^[3] ; Lima-Filho, Jose L. ^[2] ; Converti, Attilio ^[4] ; Pessoa-, Jr., Adalberto ^[1] ; Figueiredo Porto, Ana L. ^[5]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Tecnol Bioquim Farmaceut, BR-05508000 Sao Paulo - Brazil

^[2] Univ Fed Pernambuco, Lab Biotecnol, LIKA, Recife, PE - Brazil

^[3] Univ Fed Pernambuco, Dept Antibiot, Recife, PE - Brazil

^[4] Univ Genoa, CAPES Fellowship Brazil, Dept Chem & Proc Engn, I-16145 Genoa - Italy

^[5] Univ Fed Rural Pernambuco, Dept Morfol & Fisiol Anim, Dois Irmaos, PE - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 42, n. 2, p. 658-667, APR-JUN 2011.
• Citações Web of Science: 7

Resumo

Clavulanic acid is a beta-lactam antibiotic which has a potent beta-lactamase inhibiting activity. In order to optimize its production by the new isolate Streptomyces DAUFPE 3060, the influence of two independent variables, temperature and soybean flour concentration, on clavulanic acid and biomass concentrations was investigated in 250 mL-Erlenmeyers according to a 22 central composite design. To this purpose, temperature and soybean flour (SF) concentration were varied in the ranges 26-34 degrees C and 10-50 g/L, respectively, and the results evaluated utilizing the Response Surface Methodology. The experimental maximum production of clavulanic acid (629 mg/L) was obtained at 32 degrees C and 40 g/L SF after 48 h, while the maximum biomass concentration (3.9 g/L) at 30 degrees C and 50 g/L soybean flour, respectively. These values are satisfactorily close to those (640 mg/L and 3.75 g/L, respectively) predicted by the model, thereby demonstrating the validity of the mathematical approach adopted in this study. (AU)

• Processo FAPESP: 05/60158-0 - Produção e extração de ácido clavulânico de Streptomyces spp. por fermentação extrativa utilizando sistemas de duas fases aquosas
• Beneficiário: Daniela de Araújo Viana Marques
• Modalidade de apoio: Bolsas no Brasil - Doutorado