Autor(es)

Ortiz, Julia ; Marcelino, Paulo Ricardo Franco ; Teruel, Jose A. ; Aranda, Francisco J. ; Ortiz, Antonio

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 26, n. 17, p. 13-pg., 2025-09-08.

Resumo

Sophorolipids (SLs) constitute a group of unique biosurfactants in light of their unique properties, among which their physicochemical characteristics and antimicrobial activity stand out. SLs can exist mainly in acidic and lactonic forms, both of which display inhibitory activity. This study explores the interaction of non-acetylated acidic SL with bovine serum albumin (BSA). SL significantly enhances BSA's thermal stability, increasing its midpoint unfolding temperature from 61.9 degrees C to approximately 76.0 degrees C and Delta H from 727 to 1054 kJ mol-1 at high concentrations, indicating cooperative binding. Fourier-Transform Infrared Spectroscopy (FTIR) analysis confirms SL's protective effect against thermal unfolding, enabling BSA to maintain its helical structure at 70 degrees C, distinguishing it from other surfactants that cause denaturation. Furthermore, SL fundamentally alters the sequence of thermal unfolding events; beta-aggregation precedes helical domain unfolding, suggesting protective binding to BSA's helical regions. Computational docking reveals high-affinity binding (Kd = 14.5 mu M). Uniquely, SL binds between BSA domains IB and IIIA, establishing hydrophobic interactions, salt bridges, and hydrogen bonds, thus stabilizing the protein's 3D structure. This distinct binding site is attributed to SL's amphipathic character. This work deepens the understanding of the molecular characteristics of SL-protein interactions and contributes to improving the general knowledge of this outstanding biosurfactant. (AU)

• Processo FAPESP: 19/19757-0 - Avaliação da interação de biossurfactante do tipo soforolipídico com modelos de membranas fosfolipídicas
• Beneficiário: Paulo Ricardo Franco Marcelino
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
• Beneficiário: Roberto de Campos Giordano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático