Biological monitoring of a xenomaterial for grafting: an evaluation in critical-size calvarial defects

Autor(es)

Accorsi-Mendonca, Thais ^[1] ; Zambuzzi, Willian Fernando ^{[2, 3]} ; Bramante, Clovis Monteiro ^[4] ; Cestari, Tania Mari ^[5] ; Taga, Rumio ^[5] ; Sader, Marcia ^[6] ; de Almeida Soares, Gloria Dulce ^[6] ; Granjeiro, Jose Mauro ^{[7, 2, 3]}

Afiliação do(s) autor(es)

^[1] Univ Grande Rio, Dept Endodont, BR-25071202 Duque De Caxias, RJ - Brazil

^[2] Standardizat & Ind Qual INMETRO, Projects Div, Natl Inst Metrol, Rio De Janeiro - Brazil

^[3] Univ Hosp Antonio Pedro, Cell therapy Ctr, Niteroi, RJ - Brazil

^[4] Univ Sao Paulo, Dept Endodont, Bauru Dent Sch, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Dept Biol Sci, Bauru Dent Sch, Sao Paulo - Brazil

^[6] Univ Fed Rio de Janeiro, Rio De Janeiro - Brazil

^[7] Univ Fed Fluminense, Dept Cell & Mol Biol, Inst Biol, UFF, BR-24020150 Centro Niteroi, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE; v. 22, n. 4, p. 997-1004, APR 2011.
• Citações Web of Science: 5

Resumo

Our purpose was to evaluate the osteoconduction potential of mixed bovine bone (MBB) xenografts as an alternative for bone grafting of critical-size defects in the calvaria of rats. After surgery, in the time intervals of 1, 3, 6, and 9 months, rats were killed and their skulls collected, radiographed and histologically prepared for analysis. The data obtained from histological analysis reported that the particles of MBB did not promote an intense immunological response, evidencing its biocompatibility in rats. Our results clearly showed the interesting evidence that MBB was not completely reabsorbed at 9 months while a small amount of newly formed bone was deposited by osteoprogenitor cells bordering the defect. However, this discrete bone-forming stimulation was unable to regenerate the bone defect. Overall, our results suggest that the properties of MBB are not suitable for stimulating intense bone regeneration in critical bone defects in rats. (AU)

• Processo FAPESP: 04/08095-1 - Análise histomorfométrica comparativa do reparo ósseo de defeito crítico em calvária de ratos tratado com enxerto autógeno e matriz óssea desmineralizada: padrão de imunomarcação para MMP-9
• Beneficiário: Fabio Henrique Gelonese de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 01/10707-7 - Bases moleculares do controle da proliferação celular e origem de neoplasias na era de genômica e proteômica
• Beneficiário: Mari Cleide Sogayar
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 99/10655-5 - José Mauro Granjeiro | Faculdade de Odontologia de Bauru/USP - Brasil
• Beneficiário: Mari Cleide Sogayar
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Brasil