| Texto completo | |
| Autor(es): Mostrar menos - |
Silva, Maria Laina
;
Lopes, Francisco Eilton Sousa
;
Soares, Paulo Henrique Peixoto
;
Silva, Lua
;
Colares, Pedro Paulo Rodrigues
;
da Silva, Bruno Nascimento
;
Souza, Pedro Filho Noronha
;
Montenegro, Raquel Carvalho
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de Souza Collares Maia Castelo-Branco, Debora
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Lorenzon, Esteban Nicolas
;
Cilli, Eduardo Maffud
;
Carneiro, Victor Alves
;
de Aguiar Cordeiro, Rossana
Número total de Autores: 13
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| Tipo de documento: | Artigo Científico |
| Fonte: | FUTURE MICROBIOLOGY; v. N/A, p. 13-pg., 2025-10-23. |
| Resumo | |
IntroductionCandida parapsilosis has emerged among invasive fungal infections, boming an alarming problem for human health. Recent studies have focused on antimicrobial peptides and their derivatives, such as the Aurein family, as a new approach to developing cutting-edge antifungal agents.ObjectiveThis study aimed to evaluate the antifungal potential of Aurein 1.2 (Au) and two modified analogs, K-aurein (K-au) and D-aurein (D-au), containing an additional lysine or aspartic acid residue, respectively, at the N-terminal of the native peptide.Materials & MethodsTo this, antifungal activity, time of action by time-kill curve, ergosterol-binding analysis in vitro and in silico, and antibiofilm assays were performed. Results: We found that K-au demonstrated the lowest cytotoxicity and the greatest antifungal activity compared to other tested peptides. K-au showed MIC values ranging from 62.5 to 125 mu g/mL and time of action fungicide between 60 and 180 min. Molecular docking indicated strong interaction with ergosterol, particularly for K-au, supporting a membrane-targeting mechanism. Biofilm assays demonstrated that the peptides inhibited biofilm formation by up to 80% and were effective against mature biofilms, as confirmed by ultrastructural analysis.ConclusionThese findings highlight Au-derived peptides as promising molecules against C. parapsilosis. (AU) | |
| Processo FAPESP: | 13/07600-3 - CIBFar - Centro de Inovação em Biodiversidade e Fármacos |
| Beneficiário: | Glaucius Oliva |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |