Autor(es)

Silva, Vanessa Cristina Martins ; Franca, Yasmin ; de Azevedo, Lais Sampaio ; Guiducci, Raquel ; Villela, Edlaine Faria de Moura ; Luchs, Adriana

• Tipo de documento: Artigo Científico
• Fonte: Viruses-Basel; v. 17, n. 8, p. 29-pg., 2025-08-11.

Resumo

In Brazil, molecular surveillance expanded after Rotarix (TM) vaccine introduction, alongside G2P[4] dominance. The G2P[6] genotype, despite sharing the same DS-1-like constellation as G2P[4] strains, remains rare. This retrospective study analyzed eight Brazilian G2P[6] strains (2012-2014) through RT-PCR and 11-segments sequencing, followed by phylogenetic analysis. Two distinct groups were identified: 2012-2013 strains (six) carried a DS-1-like backbone with the rare NSP4 E6 genotype, while 2014 strains (two) exhibited the classical DS-1-like constellation with E2. Phylogenetic analysis confirmed the two main clusters: 2012-2013 strains related to classical G2P[4] and uncommon global genotypes, and 2014 strains resembling emerging DS-1-like G1/G3/G8P[8] reassortants. The 2012-2013 strains clustered within G2-VP7 Lineage IVa, while the 2014 strains belonged to Lineage V, reflecting the global distribution of these variants. All VP4 genes were classified within the P[6]-Ia lineage, with phylogenetic analyses suggesting separate introductions from Asia and Africa. The E6 NSP4 gene segment identified in these strains has an undetermined origin and was not previously associated with G2P[6] strains in Brazil. Despite similarities to G2P[4], G2P[6] strains remain rare, with no genomic features explaining their limited spread. Phylogenetic data indicate multiple reassortment events and international viral exchange, highlighting Brazil's role in RVA diversity. Ongoing full-genome surveillance is crucial to track rare variants and assess their public health relevance. (AU)

• Processo FAPESP: 20/14786-0 - Vigilância e epidemiologia molecular dos Bocavirus humanos associados à gastroenterite aguda
• Beneficiário: Adriana Luchs
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/09064-8 - Vigilância e epidemiologia molecular dos Bocavírus humanos associados à gastroenterite aguda
• Beneficiário: Yasmin França Viana Pires de Souza
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico