| Texto completo | |
| Autor(es): |
Silva, Priscila Kennedy
;
Silva, Jhuan Luiz
;
Sales, Antonio Augusto Borges
;
de Almeida, Maysa Barbosa
;
Vasconcelos, Caio Rafael Siqueira
;
Badoco, Fernanda Rafacho
;
Ambrosio, Sergio Ricardo
;
Rodrigues, Marcela Aldrovani
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Fitoterapia; v. 188, p. 9-pg., 2025-11-07. |
| Resumo | |
Chronic wounds, including pressure ulcers, present significant healthcare challenges due to prolonged healing, high costs, and complex pathology. Gelatin-based hydrogels infused with artepillin C (ArtC), an antioxidant compound from Brazilian green propolis, were successfully prepared and structurally characterized by Fourier-transform infrared spectroscopy-showing peaks at 3408 cm(-1) (NH stretching), 2932 cm(-1) (asymmetric CH stretching), 1655 cm(-1) (amide I), and 1238 cm(-1) (amide III)-and X-ray diffraction-revealing a broad peak at 2 theta approximate to 20 degrees and a peak at 2 theta approximate to 7-8 degrees, indicative of partial crystallinity and collagen-like triple-helix structures. Art-C was infused at 0.05 %, 0.1 %, 0.25 %, and 0.5 % concentrations into these hydrogels and applied to a murine pressure ulcer model. Results showed a dose-dependent acceleration of wound closure, with the 0.5 % formulation producing the greatest reduction in wound area (Spearman's r = -0.79, p < 0.0001), followed closely by 0.25 %. The 0.5 % hydrogel achieved superior wound contraction. Histological analysis demonstrated increased collagen deposition at both 0.25 % and 0.5 % concentrations. Additionally, reactive oxygen species and nitrite levels in wound tissues were reduced at 0.25 % and 0.5 %, confirming antioxidant activity. These results underscore the therapeutic potential of structurally characterized Art-C-infused gelatin hydrogels as an effective, cost-efficient option for chronic wound management with broad clinical applicability. (AU) | |
| Processo FAPESP: | 17/04138-8 - Realização de estudos químicos, analíticos, biológicos, farmacológicos e tecnológicos para preenchimento das lacunas no desenvolvimento do setor de própolis brasileiro |
| Beneficiário: | Jairo Kenupp Bastos |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 18/05080-6 - EMU concedido no processo 2017/04138-8 para sistema de cromatografia líquida de alta pressão semipreparativo modelo LC-20AR |
| Beneficiário: | Sergio Ricardo Ambrosio |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |