Antimicrobial and Antiviral Activities, and Biocompatibility of Titanium Coated with Electron Beam-Irradiated α-Silver Tungstate Microcrystals

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De Annunzio, Sarah Raquel ; Assis, Marcelo ; Barbugli, Paula Aboud ; Santos Rosa, Alice ; Oliveira, Thamara Kelcya Fonseca ; Quintao, Tayane Alvites Nunes ; Ferreira, Vivian Neuza Dos Santos ; Sa-Guimaraes, Thayane Da Encarnacao ; Da Conceicao, Giovanna Barbosa ; Barreto-Vieira, Debora Ferreira ; Piazza, Rodolfo Debone ; Marques, Rodrigo Fernando Costa ; Dias Miranda, Milene ; Longo, Elson ; Vergani, Carlos Eduardo

• Tipo de documento: Artigo Científico
• Fonte: ACS OMEGA; v. N/A, p. 16-pg., 2025-11-23.

Resumo

The development of biomaterials with modified surfaces is a promising strategy to prevent microbial adhesion, biofilm formation, and viral contamination on implants. Alpha-silver tungstate (alpha-Ag2WO4) exhibits well-recognized antimicrobial activity, which can be further enhanced by electron beam irradiation (EBI). This study evaluated the antimicrobial, antiviral, and cytotoxic performance of titanium (Ti) discs coated with alpha-Ag2WO4, with or without EBI treatment, using the spin coating method. Material characterization revealed no significant differences in structure, composition, or morphology between the two coatings. Both coatings increased the surface roughness and surface free energy of the discs. Regarding antibiofilm effects, reductions of 3.46 and 3.31 log1 0 CFU/mL were observed for Streptococcus sanguinis on irradiated and nonirradiated coated surfaces, respectively, whereas Candida albicans, Actinomyces naeslundii, Fusobacterium nucleatum, and Porphyromonas gingivalis showed no growth after the adhesion and biofilm formation phases. Furthermore, the alpha-Ag2WO4 coatings reduced SARS-CoV-2 titers by up to 2 log1 0 without causing cytotoxic effects in Vero E6 cells. These findings underscore the potential of alpha-Ag2WO4-based coatings as effective antimicrobial and antiviral surfaces, offering promising applications in dental implants and other biomedical devices. (AU)

• Processo FAPESP: 23/08525-7 - Desenvolvimento de curativos cutâneos 3D de colágeno marinho enriquecidos com nanoparticulas funcionais para a regeneração de feridas cutâneas
• Beneficiário: Marcelo de Assis
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 22/08487-5 - Propriedades antimicrobianas e biocompatibilidade de titânio e zircônia revestidos com ±-Ag2WO4 irradiado com feixe de elétrons
• Beneficiário: Sarah Raquel de Annunzio
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/07296-2 - CDMF - Centro de Desenvolvimento de Materiais Funcionais
• Beneficiário: Elson Longo da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs