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From Waste to Plant-Based Protein: Impact of Solid-State Fermentation on Functionality and Protein Extraction of Cassava Leaves

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Autor(es):
Miranda, Cristiane Grella ; Li, Chenghao ; Speranza, Paula ; De Oliveira, Anielle ; De Oliveira, Luciana Alves ; Tanaka, Takuji ; Nickerson, Michael T. ; Sato, Ana Carla Kawazoe
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: CEREAL CHEMISTRY; v. N/A, p. 13-pg., 2025-11-14.
Resumo

Background and Objectives Cassava leaves represent a significant waste stream in cassava cultivation, currently underutilized in both food and nonfood applications. This study investigates the use of solid-state fermentation with Aspergillus oryzae, Lactobacillus plantarum, and Bacillus subtilis to modify the composition and functional properties of cassava leaf flour (CLF), as well as to serve as a pretreatment for wet fractionation aimed at producing higher protein ingredients.Finds Results showed that fermentation increased the protein content of CLF while reducing anti-nutritional compounds. Fermentation had no significant effect on water-holding capacity or protein digestibility, but it increased oil-holding capacity and decreased solubility. Alkaline extraction followed by isoelectric precipitation was applied to extract proteins from the fermented CLF; however, fermentation did not improve protein availability, as evidenced by the lower protein purity of the concentrates when compared to non-fermented samples. Nevertheless, the functional properties of the protein concentrates obtained from the fermented cassava leaves were improved, demonstrating a positive effect of fermentation on the extracted protein's techno-functional potential.Conclusions Overall, fermentation increased the protein content of CLF, reduced anti-nutritional compounds, and enhanced the functional properties of the extracted protein.Significance and Novelty These findings suggest that fermentation of cassava leaf flour represents a viable biotechnological strategy for sustainable food industry applications, contributing to the development of more sustainable food supply chains. (AU)

Processo FAPESP: 19/27354-3 - Arquitetura de sistemas de liberação coloidais: qual é o papel da estrutura na digestibilidade?
Beneficiário:Rosiane Lopes da Cunha
Modalidade de apoio: Auxílio à Pesquisa - Temático