Spectrofluorimetric Determination of Coumarin in Commercial Tablets

Autor(es)

Marcolan, Mirian ^[1] ; Martins, Priscila Alfonso ^[1] ; Pedrosa, Valber A. ^[2] ; Rodrigues, Maira R. ^[3] ; de Oliveira, Hueder P. M. ^[4] ; Codognoto, Lucia ^[5]

Afiliação do(s) autor(es)

^[1] Univ Vale do Paraiba, Inst Pesquisa & Desenvolvimento, Sao Jose Dos Campos, SP - Brazil

^[2] UNESP, Inst Biosci, Dept Chem & Biochem, Botucatu, SP - Brazil

^[3] Polo Univ Rio das Ostras, Univ Fed Fluminense, Dept Ciencia & Tecnol, Rio Das Ostras, RJ - Brazil

^[4] Univ Camilo Castelo Branco, BR-12247004 Sao Jose Dos Campos, SP - Brazil

^[5] Univ Fed Sao Paulo, Dept Ciencias Exatas & Terra, BR-09972270 Diadema, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Fluorescence; v. 21, n. 2, p. 733-738, MAR 2011.
• Citações Web of Science: 5

Resumo

A simple, rapid and effective analytical method based on fluorescence spectroscopy for the determination of coumarin in pharmaceutical formulations without pre-treatment or pre-concentration step was development. Coumarin had maximum excitation and emission at 310 nm and 390 nm, respectively. Optimum conditions for the detection of coumarin were investigated. Under optimized conditions, we observed a linear behavior for the sign of coumarin in the concentration range of 2.5 x 10(-6) to 1.0 x 10(-4) mol L(-1), with linearity of 0.998 and sensitivity of 2.9 x 10(10) u.a/mol L(-1). The proposed method was validated in terms of accuracy, precision and specificity of coumarin using the standard addition and external calibration. It was noted that the results support (P < 0.05), indicating that the matrices were not an interference in the determination of coumarin by fluorescence spectroscopy. The results were favorable compared with those obtained by reference chromatographic methods. (AU)

• Processo FAPESP: 08/50588-6 - Eletrodos de ouro modificados por monocamadas auto-organizadas aplicados à determinação de pesticidas
• Beneficiário: Lucia Codognoto de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/56701-3 - Estudo de processos de agregacao em moleculas com potencial aplicacao em terapia fotodinamica.
• Beneficiário: Hueder Paulo Moisés de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular