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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Spectrofluorimetric Determination of Coumarin in Commercial Tablets

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Author(s):
Marcolan, Mirian [1] ; Martins, Priscila Alfonso [1] ; Pedrosa, Valber A. [2] ; Rodrigues, Maira R. [3] ; de Oliveira, Hueder P. M. [4] ; Codognoto, Lucia [5]
Total Authors: 6
Affiliation:
[1] Univ Vale do Paraiba, Inst Pesquisa & Desenvolvimento, Sao Jose Dos Campos, SP - Brazil
[2] UNESP, Inst Biosci, Dept Chem & Biochem, Botucatu, SP - Brazil
[3] Polo Univ Rio das Ostras, Univ Fed Fluminense, Dept Ciencia & Tecnol, Rio Das Ostras, RJ - Brazil
[4] Univ Camilo Castelo Branco, BR-12247004 Sao Jose Dos Campos, SP - Brazil
[5] Univ Fed Sao Paulo, Dept Ciencias Exatas & Terra, BR-09972270 Diadema, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Journal of Fluorescence; v. 21, n. 2, p. 733-738, MAR 2011.
Web of Science Citations: 5
Abstract

A simple, rapid and effective analytical method based on fluorescence spectroscopy for the determination of coumarin in pharmaceutical formulations without pre-treatment or pre-concentration step was development. Coumarin had maximum excitation and emission at 310 nm and 390 nm, respectively. Optimum conditions for the detection of coumarin were investigated. Under optimized conditions, we observed a linear behavior for the sign of coumarin in the concentration range of 2.5 x 10(-6) to 1.0 x 10(-4) mol L(-1), with linearity of 0.998 and sensitivity of 2.9 x 10(10) u.a/mol L(-1). The proposed method was validated in terms of accuracy, precision and specificity of coumarin using the standard addition and external calibration. It was noted that the results support (P < 0.05), indicating that the matrices were not an interference in the determination of coumarin by fluorescence spectroscopy. The results were favorable compared with those obtained by reference chromatographic methods. (AU)