| Texto completo | |
| Autor(es): |
Fernandes Patta, Ana C. M.
;
Mathews, Patrick D.
;
Madrid, Rafael R. M.
;
Rigoni, Vera L. S.
;
Silva, Emerson R.
;
Mertins, Omar
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | International Journal of Biological Macromolecules; v. 148, p. 15-pg., 2020-04-01. |
| Resumo | |
The production of efficient micro and nanoparticles of pH-responsive and mucoadhesive properties is of high scrutiny. We produced a drug carrier bioparticle providing such structural features. Thereby, protonated chitosan bearing chemically bonded arginine was interacted with ionized alginate, leading to the assembling of colloidal particles of specific characteristics. Ideally, the ratio of chitosan-arginine monomers to alginate monomer is 1.6 when the biopolymers are highly charged, providing electrostatic interaction with Gibbs energy compensation around similar to 14 kcal/mol. Both size and surface charge of the bioparticle respond to pH variation, leading to structures of nano to micro hydrodynamic diameters and of positive, nearly neutral and negative zeta potential, with nanoscopic structure changing from mass to surface fractals. The inclusion of two hydrophobic model drugs provided some specific physicochemical features. Following freeze-drying, the bioparticles present both irregular shape and surface morphology, but an overall similar dry structure. An in vivo study of oral administration to teleost fish revealed that the bioparticles attain the intestine mucus and further, the interaction with the intestinal mucosa is timely dependent thanks to the mucoadhesive property. The in vivo study endorsed that the bioparticle provides high compliance to freshwater ornamental fish, highlighting it as a material of promising application. (C) 2020 Elsevier B.V. All rights reserved. (AU) | |
| Processo FAPESP: | 17/16722-6 - Nanopartículas pH sensíveis para otimização da veiculação oral de antiparasitários no tratamento de peixes ornamentais |
| Beneficiário: | Ana Carolina Monge Fernandes Patta |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 18/20482-3 - Diversidade de cnidários microscópicos da Bacia Amazônica com ênfase na filogenia, sistemática e interação com seus hospedeiros |
| Beneficiário: | Patrick Mathews Delgado |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 16/24409-3 - Peptídeos Penetrantes de Células para Transporte de DNA plasmídial e microRNA: da Estrutura Nanoscópica a Entrega de Genes. |
| Beneficiário: | Emerson Rodrigo da Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 15/23948-5 - Aperfeiçoamento das propriedades do polissacarídeo quitosana para aplicação em lipossomas e vesículas gigantes |
| Beneficiário: | Omar Mertins |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 16/13368-4 - Sistemas nanoestruturados: de modelos biomiméticos de membranas a carreadores de bioativos |
| Beneficiário: | Karin Do Amaral Riske |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |