| Texto completo | |
| Autor(es): |
Costa, Bruna Z.
;
Galman, James L.
;
Slabu, Iustina
;
France, Scott P.
;
Marsaioli, Anita J.
;
Turner, Nicholas J.
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CHEMCATCHEM; v. 10, n. 20, p. 6-pg., 2018-10-23. |
| Resumo | |
A multi-enzymatic cascade process involving transaminases (TAs) and reductive aminases (RedAms) to produce enantiomerically pure 2,5-disubstituted pyrrolidine alkaloids from their respective 1,4-diketones is reported. Several TAs were screened and the best results for diketone monoamination were obtained with an R-selective TA from Mycobacterium chlorophenicum and with an S-selective TA from Bacillus megaterium. Pyrroline reduction was best performed by a reductive aminase from Ajellomyces dermatitidis (AdRedAm). Finally, a biocatalytic one-pot cascade was implemented using the aforementioned enzymes and a variety of 2-methyl-5-alkylpyrrolidines were produced with high (>99%) conversion, diastereomeric and enantiomeric excess values. (AU) | |
| Processo FAPESP: | 14/50249-8 - Green chemistry: sustainable synthetic methods employing benign solvents, safer reagents, and bio-renewable feedstock |
| Beneficiário: | Arlene Gonçalves Corrêa |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Centros de Pesquisa Aplicada |
| Processo FAPESP: | 15/22513-5 - Processos biocatalíticos envolvendo transaminases e imino redutases para a síntese de alcalóides de veneno de formiga. |
| Beneficiário: | Bruna Zucoloto da Costa |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado |
| Processo FAPESP: | 14/22967-3 - Síntese de Alcalóides Piperidínicos e Pirrolidínicos em Sistemas Multienzimáticos |
| Beneficiário: | Bruna Zucoloto da Costa |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |