| Texto completo | |
| Autor(es): Mostrar menos - |
Franca, Danilo Alves
;
Sampaio, Aryele Nunes Da Cruz Encide
;
Possebon, Fabio Sossai
;
Reis Teixeira, Wanderson Sirley
;
Ribeiro, Bruna Leticia Devide
;
Labruna, Marcelo Bahia
;
Costa, Francisco Borges
;
Sidi-Boumedine, Karim
;
Pereira, Juliano Goncalves
;
Rousset, Elodie
;
Thiery, Richard
;
Pereira Dos Santos, Fabiana Cristina
;
Colombo, Silvia
;
Megid, Jane
;
Mioni, Mateus De Souza Ribeiro
Número total de Autores: 15
|
| Tipo de documento: | Artigo Científico |
| Fonte: | VETERINARY RESEARCH COMMUNICATIONS; v. 50, n. 1, p. 8-pg., 2025-10-29. |
| Resumo | |
Coxiellosis, often asymptomatic in animals, can cause significant reproductive losses and massive shedding of Coxiella burnetii during parturition. Serological surveillance is essential for identifying positive herds and implementing control measures to protect animal and human health. The World Organization for Animal Health (WOAH) recommends the enzyme-linked immunosorbent assay (ELISA) for animal serosurveys due to its high sensitivity and operational simplicity. However, in many countries, the absence of authorized commercial kits and the high cost of imports limit both epidemiological surveillance and clinical diagnosis. This study evaluated the diagnostic performance of an in-house immunofluorescence assay (IFA) compared with a commercial ELISA kit (IDVet ID Screen Q fever indirect multi-species). In a set of 182 bovine sera, the in-house IFA achieved a sensitivity of 84.78%, specificity of 98.89%, and diagnostic accuracy of 91.76%, with a Cohen's kappa coefficient of 0.835 (95% CI: 0.692-0.979), indicating almost perfect agreement. The positive predictive value (PPV) was 98.73%, and the negative predictive value (NPV) was 86.41%. The moderate positive correlation between IFA titers and ELISA S/P% values (rho = 0.503; p < 0.0001) supports their overall concordance despite differences in antigen origin. These results indicate that the in-house IFA is a reliable alternative for diagnosing and conducting epidemiological surveys of C. burnetii infection in cattle, particularly in settings where no commercial kit is available. Its high specificity and PPV make it especially valuable for confirming infection status in surveillance programs. (AU) | |
| Processo FAPESP: | 24/23019-3 - Febre Maculosa Brasileira e Febre Q em comunidades indígenas e quilombolas dos Estados de São Paulo e Rio de Janeiro: uma abordagem de Saúde Única |
| Beneficiário: | Danilo Alves de França |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 19/05300-9 - Isolamento, caracterização e sequenciamento genético de estirpes de Coxiella burnetii do Brasil |
| Beneficiário: | Mateus de Souza Ribeiro Mioni |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |