Sugarcane genes differentially expressed during water deficit

Autor(es)

Rodrigues, F. A. ^[1] ; Da Graca, J. P. ^[1] ; De Laia, M. L. ^{[2, 3]} ; Nhani-, Jr., A. ^[4] ; Galbiati, J. A. ^[5] ; Ferro, M. I. T. ^[2] ; Ferro, J. A. ^[2] ; Zingaretti, S. M. ^{[1, 6]}

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Brazilian Clone Collect Ctr, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal - Brazil

^[2] UNESP Univ Estadual Paulista, Dept Technol, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal - Brazil

^[3] Univ Fed Vales Jequitinhonha & Mucuri, FCA, Dept Florestal Engn, BR-39100000 Diamantina, MG - Brazil

^[4] Embrapa Trigo, Ctr Biotechnol, BR-99001970 Passo Fundo, RS - Brazil

^[5] UNESP Univ Estadual Paulista, Dept Rural Engn, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal - Brazil

^[6] Univ Ribeirao Preto, Unit Biotechnol, BR-14096900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Biologia Plantarum; v. 55, n. 1, p. 43-53, MAR 2011.
• Citações Web of Science: 19

Resumo

To identify genes that are up and down-regulated by water deficit in sugarcane we used the macroarray methodology and the expression level of 3 575 independent sugarcane cDNAs was measured by hybridization with RNA extracted from plants submitted to mild, moderate and severe water deficit. We identified approximately 1 670 differentially expressed genes from which 62 % were up-regulated by different stress-conditions, whereas many repressed genes were exclusive for each time-point. Analysis of similarity showed that approximately 24 % of the differentially expressed genes shared homology with proteins involved in different processes such as signal transduction, hormone metabolism, photosynthesis, transcription and stress response. Transcripts with no known function accounted for approximately 39 % and those without similarity represented 36 % of the sequences. Five genes analyzed by RT-PCR confirmed the macroarray results. (AU)

• Processo FAPESP: 02/04600-8 - Identificação de etiquetas de sequências expressas (ESTs) e genes relacionados ao mecanismo de tolerância ao estresse hídrico em raízes e folhas de plântulas de cana-de-açúcar
• Beneficiário: Sonia Marli Zingaretti
• Modalidade de apoio: Auxílio à Pesquisa - Regular