Different air-water spray regulations affect the healing of Er,Cr:YSGG laser incisions

Autor(es)

Sperandio, Felipe Fornias ^{[1, 2]} ; Meneguzzo, Daiane T. ^[3] ; Ferreira, Leila S. ^[1] ; da Ana, Patricia A. ^[3] ; Azevedo, Luciane H. ^[1] ; de Sousa, Suzana C. O. M. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent, LELO, BR-05508900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Dent, Dept Oral Pathol, BR-05508900 Sao Paulo - Brazil

^[3] IPEN CNEN SP, Ctr Lasers & Aplicacoes, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 26, n. 2, p. 257-265, MAR 2011.
• Citações Web of Science: 6

Resumo

Surgeries performed with high-intensity laser devices may be improved with accurate protocols, including the air-water spray regulation. Thus, this study sought to investigate the healing process of wounds made on the dorsum of rat tongues using an Er,Cr:YSGG laser device with different air-water spray regulations. The incisions were made on the dorsum of Wistar rat tongues using an Er,Cr:YSGG laser with three different air-water spray regulations (100/0%, 50/50%, 11/7%). Scalpel incisions functioned as controls. The sacrifices occurred between 0 and 14 days after surgery. Morphological, histological, and immunohistochemical (fibronectin and type III collagen) analysis of the wounds were performed. The air-water spray regulation influenced wound healing and the inflammatory response, especially in the earlier stages. Incisions performed using the 100/0% air/water spray regulation had the worst results, expressing a greater amount of fibronectin and type III collagen. The 50/50% air/water spray regulation brought in a non-clear surgical field and poor laser interaction with the tissue. The 11/7% air/water spray regulation showed the best clinical results and less pronounced histological events. According to the results encountered, the air-water spray should be regulated to improve surgery. (AU)

• Processo FAPESP: 04/06794-0 - Processo reparacional em tecido cutâneo e oral de ratos submetidos a incisão cirúrgica com lasers de CO2 e diodo, bisturi elétrico e convencional: uma análise morfológica, histoquímica e imuno-histoquímica
• Beneficiário: Suzana Cantanhede Orsini Machado de Sousa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/60243-8 - Avaliação da reparação tecidual de incisões realizadas com o laser de ER, Cr:YSGG em dorso lingual de ratos
• Beneficiário: Felipe Fornias Sperandio
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica