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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Different air-water spray regulations affect the healing of Er,Cr:YSGG laser incisions

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Author(s):
Sperandio, Felipe Fornias [1, 2] ; Meneguzzo, Daiane T. [3] ; Ferreira, Leila S. [1] ; da Ana, Patricia A. [3] ; Azevedo, Luciane H. [1] ; de Sousa, Suzana C. O. M. [2]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Sch Dent, LELO, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Dent, Dept Oral Pathol, BR-05508900 Sao Paulo - Brazil
[3] IPEN CNEN SP, Ctr Lasers & Aplicacoes, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Lasers in Medical Science; v. 26, n. 2, p. 257-265, MAR 2011.
Web of Science Citations: 6
Abstract

Surgeries performed with high-intensity laser devices may be improved with accurate protocols, including the air-water spray regulation. Thus, this study sought to investigate the healing process of wounds made on the dorsum of rat tongues using an Er,Cr:YSGG laser device with different air-water spray regulations. The incisions were made on the dorsum of Wistar rat tongues using an Er,Cr:YSGG laser with three different air-water spray regulations (100/0%, 50/50%, 11/7%). Scalpel incisions functioned as controls. The sacrifices occurred between 0 and 14 days after surgery. Morphological, histological, and immunohistochemical (fibronectin and type III collagen) analysis of the wounds were performed. The air-water spray regulation influenced wound healing and the inflammatory response, especially in the earlier stages. Incisions performed using the 100/0% air/water spray regulation had the worst results, expressing a greater amount of fibronectin and type III collagen. The 50/50% air/water spray regulation brought in a non-clear surgical field and poor laser interaction with the tissue. The 11/7% air/water spray regulation showed the best clinical results and less pronounced histological events. According to the results encountered, the air-water spray should be regulated to improve surgery. (AU)