Dressing liposomal particles with chitosan and poly(vinylic alcohol) for oral vaccine delivery

Autor(es)

Rescia, Vanessa C. ^{[1, 2]} ; Takata, Celia S. ^[3] ; de Araujo, Pedro S. ^[4] ; Bueno da Costa, Maria H. ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Microesferas & Lipossomas, Ctr Biotecnol, BR-05503900 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Disciplina Clin Med, Dept Med, Sao Paulo - Brazil

^[3] Inst Butantan, Div Desenvolvimento Tecnol & Prod, BR-05503900 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Dept Bioquim IQ, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Liposome Research; v. 21, n. 1, p. 38-45, MAR 2011.
• Citações Web of Science: 17

Resumo

Liposomes have been used as adjuvants since 1974. One major limitation for the use of liposomes in oral vaccines is the lipid structure instability caused by enzyme activities. Our aim was to combine liposomes that could encapsulate antigens (i.e., Dtxd, diphtheria toxoid) with chitosan, which protects the particles and promotes mucoadhesibility. We employed physical techniques to understand the process by which liposomes (SPC: Cho, 3: 1) can be sandwiched with chitosan (Chi) and stabilized by PVA (poly-vinylic alcohol), which are biodegradable, biocompatible polymers. Round, smooth-surfaced particles of REVs-Chi (reversed-phase vesicles sandwiched by Chi) stabilized by PVA were obtained. The REVs encapsulation efficiencies (Dtxd was used as the antigen) were directly dependent on the Chi and PVA present in the formulation. Chi adsorption on the REVs surface was accompanied by an increase of zeta-potential. In contrast, PVA adsorption on the REVs-Chi surface was accompanied by a decrease of zeta-potential. The presence of Dtxd increased the Chi surface-adsorption efficiency. The PVA affinity by mucine was 2,000 times higher than that observed with Chi alone and did not depend on the molecule being in solution or adsorbed on the liposomal surface. The liberation of encapsulated Dtxd was retarded by encapsulation within REVs-Chi-PVA. These results lead us to conclude that these new, stabilized particles were able to be adsorbed by intestinal surfaces, resisted degradation, and controlled antigen release. Therefore, REVs-Chi-PVA particles can be used as an oral delivery adjuvant. (AU)

• Processo FAPESP: 00/10970-7 - Microencapsulamento de macromoléculas em polímeros biodegradáveis
• Beneficiário: Maria Helena Bueno da Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular