Fipronil-induced cell death in salivary glands of Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) semi-engorged females

Autor(es)

Martins Pereira, Carolina Parga ^[1] ; de Oliveira, Patricia Rosa ^[1] ; Scopinho Furquim, Karim Christina ^[1] ; Bechara, Gervasio Henrique ^[2] ; Camargo-Mathias, Maria Izabel ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Biol, BR-13506900 Rio Claro, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Fac Agron & Vet Sci, Dept Anim Pathol, BR-14884900 Jaboticabal, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Experimental Parasitology; v. 127, n. 2, p. 481-489, FEB 2011.
• Citações Web of Science: 11

Resumo

The tick Rhipicephalus sanguineus is currently considered an urban plague. For this reason many studies are intended to find methods to control these ectoparasites. Thus, the present study analyzed the ultrastructural modifications of the salivary glands cells of semi-engorged females of R. sanguineus resulting from their exposition to Fipronil (active ingredient of Frontline (R)). The studied individuals were divided into four groups. Group 1 was exposed to distilled water (control) and groups 2, 3 and 4 were exposed to 1, 5 and 10 ppm of Fipronil, respectively. The salivary gland of ticks subjected to the acaricide showed accelerated process of cell death by atypical apoptosis, as well as augmented cell damages as the concentration of the chemical compound was increased. The acaricide toxicity at cellular level was demonstrated by remarkable changes of elements of the cytoskeleton and spherocrystals (extremely hard inorganic structures). However, tick defense mechanisms, such as the observed autofagic vacuoles proved the cells attempt to preserve their integrity and minimize the devastating action of this chemical compound on the salivary glands. (C) 2010 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 07/58633-8 - Analise dos efeitos do fipronil (ingrediente ativo do frontline) nas celulas das glandulas salivares de femeas de rhipicephalus sanguineus (latreille, 1806) (acari: ixodidae).
• Beneficiário: Carolina Parga Martins Pereira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 06/52599-0 - Avaliação dos efeitos do fipronil (ingrediente ativo do Frontline) em ovários de carrapatos Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) e na medula óssea de roedores
• Beneficiário: Patrícia Rosa de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Doutorado