Involvement of an Alternative Oxidase in Oxidative Stress and Mycelium-to-Yeast Differentiation in Paracoccidioides brasiliensis

Autor(es)

Martins, Vicente P. ^[1] ; Dinamarco, Taisa M. ^[1] ; Soriani, Frederico M. ^[1] ; Tudella, Valeria G. ^[1] ; Oliveira, Sergio C. ^[2] ; Goldman, Gustavo H. ^{[3, 4]} ; Curti, Carlos ^[5] ; Uyemura, Sergio A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Sao Paulo - Brazil

^[2] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, BR-31270901 Belo Horizonte, MG - Brazil

^[3] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Farmaceut, BR-14040903 Sao Paulo - Brazil

^[4] Lab Nacl Ciencia & Tecnol Bioetanol CTBE, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Quim & Fis, BR-14040903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Eukaryotic Cell; v. 10, n. 2, p. 237-248, FEB 2011.
• Citações Web of Science: 37

Resumo

Paracoccidioides brasiliensis is a thermodimorphic human pathogenic fungus that causes paracoccidioidomycosis (PCM), which is the most prevalent systemic mycosis in Latin America. Differentiation from the mycelial to the yeast form (M-to-Y) is an essential step for the establishment of PCM. We evaluated the involvement of mitochondria and intracellular oxidative stress in M-to-Y differentiation. M-to-Y transition was delayed by the inhibition of mitochondrial complexes III and IV or alternative oxidase (AOX) and was blocked by the association of AOX with complex III or IV inhibitors. The expression of P. brasiliensis aox (Pbaox) was developmentally regulated through M-to-Y differentiation, wherein the highest levels were achieved in the first 24 h and during the yeast exponential growth phase; Pbaox was upregulated by oxidative stress. Pbaox was cloned, and its heterologous expression conferred cyanide-resistant respiration in Saccharomyces cerevisiae and Escherichia coli and reduced oxidative stress in S. cerevisiae cells. These results reinforce the role of PbAOX in intracellular redox balancing and demonstrate its involvement, as well as that of other components of the mitochondrial respiratory chain complexes, in the early stages of the M-to-Y differentiation of P. brasiliensis. (AU)

• Processo FAPESP: 99/04126-0 - Estudos moleculares bioenergéticos e regulação intracelular de cálcio no fungo sistêmico Paracoccidioides brasiliensis
• Beneficiário: Sergio Akira Uyemura
• Modalidade de apoio: Auxílio à Pesquisa - Regular